Objective:To study the aberrant methylation in putative promoter region of MicroRNA mir219 and the methylation correlated expression down-regulation in HCC(hepatocellular carcinoma), and explore the potential role of mir219 in HCC. Methods:In HCC tumor sample and cell line, the methylation status of putative promoter region of mir219 by pyrosequence and TA sequencing were detected; and the expression detected by Taqman real time RT-PCR. Results:There were aberrant methylation in the CpG Island 1kb upstream and stem-loop sequence of mir219 in HCC, and the mir219 transcription was also significant down-regulated in HCC cell line. Conclusion:The 1094 bp upstream and stem-loop sequence was putative promoter region of mir219, which was aberrantly methylated in HCC and transcription down-regulated in HCC. It indicated its potential role in HCC development.