皮下注射的骨髓间充质干细胞在正常小鼠体内的迁移分化
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江苏省自然科学基金重点资助(BK2006729)


The migration and differentiation of bone marrow mesenchymal stem cells in normal mice in vivo
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    摘要:

    目的:探讨骨髓间充质干细胞(BM-MSCs)在正常小鼠体内从皮下向不同器官迁移的能力-方法:从β半乳糖苷酶(β-gal+)转基因小鼠分离骨髓细胞,经贴壁和传代培养获得第3代β-gal+ BM-MSCs,皮下注射给正常6周龄C57BL/6J野生型小鼠-8周后通过LacZ染色示踪β-gal+ BM-MSCs在受体小鼠不同组织器官的分布,并对供体第3代β-gal+ BM-MSCs 基质金属蛋白酶(MMPs)的基因表达水平进行检测-结果:β-gal转基因小鼠来源的供体第3代BM-MSCs呈现LacZ 染色阳性-第3代的β-gal阳性 BM-MSCs皮下注射8周后,在受体小鼠肺-脑-心-肝-肾内检测到LacZ 染色阳性的细胞-第3代β-gal+ BM-MSCs表达MMPs基因-结论:皮下注射的BM-MSCs能够在正常小鼠体内经血液循环迁移到不同器官,并可能分化成为不同器官的终末分化细胞,参与正常组织的自我更新-

    Abstract:

    Objective:To determine whether bone marrow mesenchymal stem cells(BM-MSCs) can migrate subcutaneously into different organs. Methods:The bone marrow cells isolated from β-galactosidase transgenic mice were cultured. BM-MSCs were passaged until the 3rd generation. The 3rd passaged BM-MSCs derived from β-galactosidase transgenic mice were transplanted into 6-week-old C57BL/6J wild type mice subcutaneously. 8 weeks later,β-gal+ MSCs were shown the distribution in various organs from recipient mice through LacZ staining. The expression of matrix metalloproteinase(MMPs) genes in the 3rd passaged β-gal+ BM-MSCs were examined by real-time RT-PCR. Results:The 3rd passaged BM-MSCs derived from β-galactosidase transgenic mice were positive for LacZ staining. Followed subcutaneous injection of the 3rd passaged β-gal+ BM-MSCs for 8 weeks,β-gal positive organ specific cells were detected in lung,brain,heart,liver and kidney from recipient mice. MMPs genes were expressed in the 3rd passaged β-gal+ BM-MSCs. Conclusion:BM-MSCs transplanted through subcutaneously can migrate into various organs through blood circulation and may differentiate into various organ specific cells to participate in self-renewal of normal tissues.

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靳建亮,苗登顺.皮下注射的骨髓间充质干细胞在正常小鼠体内的迁移分化[J].南京医科大学学报(自然科学版),2009,29(5):613-617

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  • 收稿日期:2009-01-13
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