溃疡性结肠炎小鼠结肠Cajal间质细胞及干细胞因子的变化
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江苏省自然科学基金资助(BK2008466)


Expression of interstitial cells of Cajal and stem cell factors in mice colon with ulcerative colitis
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    摘要:

    目的:探讨结肠Cajal间质细胞(ICC)-干细胞因子(SCF)在溃疡性结肠炎(UC)发病中的作用-方法:将BALB/c小鼠随机分为正常对照组(n = 8)和UC组(n = 24),于造模后1-4-7天分别处死UC组8只小鼠,取远端结肠,用Western blot方法检测SCF的表达;免疫组化检测结肠ICC的变化,并进行结肠组织学评分-结果:结肠组织学评分在7天达最高值(P < 0.05)-与正常小鼠比较,UC小鼠结肠肌间ICC表达明显减少(P < 0.05),结肠SCF的表达也显著减少(P < 0.01),SCF与ICC呈正相关(UC 4天时,r = 0.862,P = 0.027;UC 7天时,r = 0.948,P = 0.004)-结论:UC小鼠远端结肠组织内SCF表达下降,伴随ICC数量减少,提示UC病变可能与SCF/Kit系统下调有一定的相关性-

    Abstract:

    Objective:To investigate the expression of interstitial cells of Cajal(ICC)and stem cell factor(SCF)in mice colon with ulcerative colitis(UC). Methods:Thirty-two male health adult BALB/c mice were divided randomly into control group and UC groups. Eight mice of the UC groups were sacrificed after 1,4 and 7 days respectively.The colon histological score was made according to Dieleman’s method,and the expression of ICC and SCF in distal colon was detected by immunohischemistry and Western blot analysis. Results:Comparing with normal mice,the colon histological score showed a peak level at 7th day(P < 0.05). The number of distal colon ICC in UC groups was more significantly decreased than that in the normal mice(P < 0.05). Meanwhile,the expression of SCF of distal colon in UC groups was significantly decreased in UC groups compared with that in normal mice(P < 0.01). There were high positive correlations between SCF and ICC(UC 4 d,r = 0.862,P = 0.027;UC 7 d,r = 0.948,P = 0.004). Conclusion:The decrease of SCF and ICC in the distal colon of mice with ulcerative colitis may be an effective factor which is associated with the mechanism of ulcerative colitis.

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李 慧,陆宗海,陈 蕾,林 琳.溃疡性结肠炎小鼠结肠Cajal间质细胞及干细胞因子的变化[J].南京医科大学学报(自然科学版),2009,29(5):648-651

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  • 收稿日期:2008-12-30
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