实时荧光定量RT-PCR检测结肠癌中Musashi-1 mRNA的表达
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江苏省卫生厅科研基金项目(H200846,H2009)


The mRNA expression of Musashi-1 gene in clonic cancer by quantitative real-time fluorescence RT-PCR
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    摘要:

    目的:了解正常结肠干/祖细胞标志Musashi-1mRNA在结肠癌中的表达情况-方法:应用实时荧光相对定量RT- PCR的方法检测25例结肠癌组织和18例远端正常结肠组织中Musashi-1mRNA的表达,用2-△△CT的方法计算相对于远端正常组织结肠癌组织中Musashi-1mRNA的表达倍数-结果:经实时荧光定量RT-PCR 法检测,结肠癌组织中Musashi-1mRNA的表达与远端正常结肠组织相比有差异(P < 0.01),且是远端正常结肠组织的4.24倍(1.95~9.23)-低分化组织与高-中分化组织相比,Musashi-1mRNA的表达有差异(分别为P < 0.001-P < 0.005)-低分化组织中Musashi-1的表达是高分化组织的5.54倍(3.07~9.98)-结论:正常肠干/祖细胞标志物Musashi-1在结肠癌组织中尤其是低分化组织中的高表达提示Musashi-1可能在结肠肿瘤的发生发展中起着一定的作用-

    Abstract:

    Objective:To explore the expression of Musashi-1 mRNA in colonic cancer,which is a putative intestinal stem and early progenitor cell marker. Methods:The mRNA expression of Musashi-1 gene in 25 colonic cancer specimens and 18 distal normal colon tissues was detected by relatively quantitative real-time RT-PCR method. The multiples of expression of Musashi-1 in colonic cancer relative to distal normal colon tissue was calculated by the method of 2-△△CT. Results:By Real-time RT-PCR method,the expression of Musashi-1 mRNA in colonic cancer was 4.24(1.95-9.23)times higher than that in distal normal colon tissue. There was a significant difference between them(P < 0.01). The expression of Musashi-1 mRNA in the poorly differentiated colonic cancer was significantly different from the high and medium differentiated tissues(P < 0.001 and P < 0.005). The expression of Musashi-1 mRNA in the poorly differentiated colonic cancer was 5.54(3.07-9.98)times higher than that in the high differentiated tissues. Conclusion:The expression of Musashi-1 mRNA in colonic cancer especially is higher in poorly differentiated tissues than in distal normal colon tissue,which suggests that Musashi-1,the intestinal stem and early progenitor cell marker,may play an important role in the initiation and development of the colonic cancer.

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朱敬华,周建农.实时荧光定量RT-PCR检测结肠癌中Musashi-1 mRNA的表达[J].南京医科大学学报(自然科学版),2009,29(5):656-659668

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  • 收稿日期:2009-01-03
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