福莫特罗和ICI118551对大鼠骨髓间充质干细胞来源的成骨样细胞功能的影响
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:


The effects of Formoterol and ICI118551 on rat osteoblast-like cells in vitro induced from marrow-derived mesenchymal stem cells
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:研究选择性β2肾上腺素能激动剂福莫特罗(Formoterol)和阻滞剂ICI118551对体外由大鼠骨髓间充质干细胞(MMSC)成骨分化的影响,探讨β2肾上腺素能受体信号对骨代谢的影响-方法:取3周龄雌性SD大鼠,全贴壁筛选法培养MMSC,用条件培养液诱导向成骨细胞分化后分别加入不同浓度Formoterol和ICI118551,检测细胞碱性磷酸酶,骨钙素的分泌水平和细胞增殖率-结果:MMSC成功诱导分化为成骨样细胞,不同浓度(10-5~10-9 mmol/L)的Formoterol均可抑制成骨样细胞分泌碱性磷酸酶,骨钙素和细胞增殖-高浓度ICI118551(10-5和 10-6 mmol/L)抑制成骨样细胞分泌碱性磷酸酶,骨钙素和细胞增殖,低浓度ICI118551(10-8 和10-9 mmol/L)则可促进成骨样细胞分泌碱性磷酸酶,骨钙素和细胞增殖-结论:β2肾上腺素能受体激动剂可抑制体外成骨样细胞的功能和细胞增殖,而阻滞剂对成骨样细胞功能和增殖的影响与其浓度有关-

    Abstract:

    Objective:To evaluate the effects of Formoterol(β2-adrenergic receptor-specific agonist) and ICI118551(β2-adrenergic receptor-specific antagonist) on rat osteoblast-like cells induced from marrow-derived mesenchymal stem cells(MMSC). Methods:MMSCs by whole bone marrow adherent culture derived from a three-week-old female SD rat are induced to osteoblast-like cells. After different concentrations of Formoterol and ICI118551 are added into the culture medium,thealkali phosphatase and osteocalcin is detected. Results:The alkali phosphatase,osteocalcin and proliferation of cells cultured with all different concentrations(10-5-10-9 mmol/L) of Formoterol are obviously low. As to ICI118551,high concentrations(10-5 and 10-6 mol/L) can inhibit the expression of the alkali phosphatase,osteocalcin and proliferation of cells while low concentrations(10-8 and 10-9 mol/L) can enhance the expression of the alkali phosphatase,osteocalcin and proliferation. Conclusion:The β2-adrenergic receptor-specific agonist can inhibit the activity of rat osteoblast-like cells in vitro while the effects of the β2-adrenergic receptor-specific antagonist are not single,depending on its concentration.

    参考文献
    相似文献
    引证文献
引用本文

胡安全,余利鹏,韦永中.福莫特罗和ICI118551对大鼠骨髓间充质干细胞来源的成骨样细胞功能的影响[J].南京医科大学学报(自然科学版),2009,29(6):784-789

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2008-12-11
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明