冠心病患者外周血CD4+ Foxp3+ 调节性T细胞与细胞因子水平及临床意义
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Level of CD4+ Foxp3+ regulatory T cells and cytokines in peripheral blood of patients with coronary heart disease and its clinic significance
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    目的:探讨不同类型冠心病患者外周血CD4+ Foxp3+ 调节性T细胞(CD4+ Foxp3+ Treg)与细胞因子的变化及临床意义-方法:分别采用流式细胞术和酶联免疫吸附法(ELISA)检测21例急性心肌梗死-16例不稳定型心绞痛-21例稳定型心绞痛患者外周血CD4+ Foxp3+ Treg百分率及肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)-白介素-10(interleukin-10,IL-10)-干扰素γ(interferon γ,IFN-γ)-白介素-4(interleukin-4,IL-4)-转化生长因子β(transforming growth factor β,TGF-β)的表达水平-结果:①急性冠脉综合征患者外周血CD4+ Foxp3+ Treg百分率显著低于正常人(P < 0.001);②急性心肌梗死患者血浆TNF-α表达水平显著高于稳定型心绞痛患者(P < 0.05),其CD4+ Foxp3+ Treg百分率与血浆IL-10水平呈显著正相关(r = 0.751,P =0.005);③冠心病患者外周血CD4+ Foxp3+ Treg百分率与血浆TNF-α-IFN-γ-IL-4及TGF-β表达水平无显著相关性;④冠心病患者外周血CD4+ Foxp3+ Treg百分率与血清总胆固醇-甘油三酯-高密度脂蛋白-低密度脂蛋白无显著相关性(P > 0.05)-结论:CD4+ Foxp3+ Treg的下降和TNF-α的升高可能参与急性冠脉综合征的发生发展-

    Abstract:

    Objective:To investigate the expression of CD4+ Foxp3+ regulatory T cells and different cytokines in peripheral blood of patients with coronary heart disease(CHD),and to explore the roles of them in CHD pathogenesis. Methods:21 patients with acute myocardial infarction(AMI),16 patients with unstable angina(UA),21 patients with stable angina(SA)were recruitde. The percentage of CD4+ Foxp3+ Treg of patients was measured by flow cytometry,and ELISA was used to analyze the blood plasma levels of cytokines(TNF-α,IL-10,IFN-γ,IL-4,TGF-β). Results:①The percentage of CD4+ Foxp3+ Treg decreased significantly in patients with acute coronary syndrome(ACS)compared with normal group(P < 0.001). ②Compared with SA patients,the level of TNF-α in AMI patients significantly increased(P < 0.05). In addition,the percentage of CD4+ Foxp3+ Treg in AMI patients was positively correlated with level of IL-10(P < 0.05). ③No correlation was found between CD4+ Foxp3+ Treg percentage and other cytokines(TNF-α,IFN-γ,IL-4,TGF-β)in CHD patients. ④There was no correlation between CD4+ Foxp3+ Treg and levels of total cholesterol,triglyceride,high or low density lipoprotein. Conclusion:The decreased percentage of CD4+ Foxp3+ Treg and increased TNF-α in peripheral blood of ACS patients may participate in ACS development and progression.

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应单平,徐 标.冠心病患者外周血CD4+ Foxp3+ 调节性T细胞与细胞因子水平及临床意义[J].南京医科大学学报(自然科学版),2009,29(6):811-814

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  • 收稿日期:2008-12-26
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