GFAP?NF在原发性脑干损伤后的表达及诊断价值分析
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南京医科大学科技发展基金重点资助项目(06NMUZ040)


The expression and diagnostic value analysis of GFAP and NF after primary brain stem injury
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    摘要:

    目的:研究原发性脑干损伤后胶质纤维酸性蛋白(GFAP)-神经丝蛋白(NF)染色的表达变化及其诊断价值-方法:收集明确死于原发性脑干损伤和脑外疾病者共65例,分为损伤组(25例)和对照组(其中死于心血管疾病20例-非心血管疾病20例),通过对中脑-脑桥和延脑切片,HE染色光镜下观察,免疫组化GFAP- NF染色观察,对所得数据作统计学处理-结果:损伤组中脑干出现星形细胞的急性反应及神经轴索的特征性改变,损伤组与对照组相比,脑干的星形细胞数量及轴索肿胀程度有明显差异(P<0.05)- 结论:上述病理变化对原发性脑干损伤的尸解病理诊断有一定的价值,通过测量星形细胞数量及轴突横径,为脑干损伤导致死亡的诊断提供了一种可参考的量化指标-

    Abstract:

    Objective:To study the expression and diagnostic value of staining glial fibrillary acidic protein(GFAP) and neurofilament protein(NF) after primary brain-stem injury. Methods:The cases that definitely died of the primary brain-stem injury and other disease were selected randomly(65 cases). All cases were divided into experimental group(25 cases) and control group(20 cases died of cardiovascular disease and 20 cases died of other disease).The brain-stem tissues were cut and stained by hematoxylin-eosin and immunohistochemical GFAP and NF,the data were analyzed. Results:Compared with the control group,astrocyte appeared acute reaction and axon showed distinctive changes in brain-stem tissues. There were significant differences in astrocyte quantity and axonal diameter of brain-stem tissue between the experimental group and the control group(P < 0.05). Conclusion:The above histopathologic changes might be valuable for autopsy pathological diagnosis of primary brain-stem injury. With the measurement of astrocyte quantity and axonal diameter,we provide a referenced quantization index for diagnosis of primary brain-stem injury.

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杨树东,孙荣超,周志毅,梁加贝,芮 俊. GFAP?NF在原发性脑干损伤后的表达及诊断价值分析[J].南京医科大学学报(自然科学版),2009,29(6):820-824

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  • 收稿日期:2009-01-15
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