齐墩果酸预处理对大鼠肝脏缺血再灌注时糖原含量的影响
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国家自然科学基金资助项目(30672523)


Effects of pretreatment with oleanolic acid on glycogen content in rats during hepatic ischemic reperfusion
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    摘要:

    目的:探讨齐墩果酸(OA)预处理对大鼠肝脏缺血再灌注(IR)时糖原含量的影响及其意义-方法:128只雄性SD大鼠随机均分为假手术组(A组),IR组(B组),0.5%羧甲基纤维素钠组(CMC-Na)(C组),0.5%CMC-Na和OA的混悬液组(D组)-术前D组以100 mg/kg的OA混悬液,A-B组以相同容积水,C组以相同容积的单纯0.5%CMC-Na分别每日灌胃1次,连续7日-第8日建立70%肝脏IR(缺血60 min后行再灌注)模型,分别测定术前,再灌注0-3-6 h血清丙氨酸转移酶(ALT)活性-肝组织的糖原含量和乳酸值,并在光镜下观察肝组织病理学改变-结果:术前D组糖原含量分别高于其他各组,差异有显著性(P < 0.05),再灌注0-3-6 h,B-C-D组糖原含量分别低于A组,差异有显著性(P < 0.05),但D组糖原含量分别高于B和C组,差异有显著性(P < 0.05)-再灌注0 h D组乳酸值分别高于其他各组,差异有显著性(P < 0.05);B和C组乳酸值分别高于A组,差异有显著性(P < 0.05)-再灌注3-6 h,B-C-D组ALT活性均高于A组,差异有显著性(P < 0.05);D组ALT活性分别低于B和C组,差异有显著性(P < 0.05);D组肝脏病理形态学改变分别较B-C组减轻-结论:OA预处理可增加大鼠肝脏的糖原含量,减轻肝脏IR时的损伤,其机制可能与增加IR时的能量供给有关-

    Abstract:

    Objective:To investigate the effect of pretreatment with oleanolic acid(OA) on glycogen content during hepatic ischemic reperfusion(IR)in rats. Methods:128 Male Sprague-Dawley(SD)rats were randomly devided into sham(A),IR(B),0.5%sodium carboxymethycellulose(CMC-Na)(C),OA+0.5% CMC-Na(D)equally. Before operation,all rats got intragastric administration once a day for 7 days, group D with OA(100 mg/kg)+0.5% CMC-Na,group A,B with water,group C with 0.5% CMC-Na in same volume. At the 8th day,rats suffered from segmental(70%)hepatic ischemia for 60 min and were followed by different periods of reperfusion. Blood and liver tissues were collected and measured respectively at preoperation,0,3 and 6 h after reperfusion for ALT,glycongen and lactate. And changes of histological pathology were also observed by light microscope. Results:Before operation,glycongen content of group D increased compared with other groups(P < 0.05). After reperfusion glycogen content in grup A decreased at 0,3,6 h compared with group B,C and D(P < 0.05);glycongen content in group D increased compared with group B and C respectively(P < 0.05). At 0 h after reperfusion,lactate level of group D increased compared with other groups(P < 0.05);lactate level of group B and C increased compared with group A(P < 0.05). At 3,6 h after reperfusion,the activity of ALT of group B,C and D increased compared with group A(P < 0.05);the activity of ALT in group D decreased compared with group B and C respectively(P < 0.05);group D had relieved morphologic changes compared with group B and C. Conclusion:OA pretreatment could increase glycogen content,also provide the rat liver with significant tolerance to IR injury. The mechanism may be related to the increased level of energy during IR.

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许仄平,张 杰,钱燕宁,孙宏斌,陆 森.齐墩果酸预处理对大鼠肝脏缺血再灌注时糖原含量的影响[J].南京医科大学学报(自然科学版),2009,29(7):930-933

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  • 收稿日期:2009-02-10
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