抑胃多肽主动免疫治疗对大鼠行为活动的影响
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国家“十一五”科技支撑课题(2006BAI02B08)


Effects of active immunization against gastric inhibitory popeptide(GIP)on behavior in rats
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    摘要:

    目的:观察抑胃多肽(GIP)靶向的主动免疫干预治疗对大鼠行为学的影响-方法:高脂喂养的SD大鼠分为实验组及对照组-实验组如期皮下多点注射GIP靶向的人工抗原(GIP-KLH);对照组如期皮下多点注射KLH-检测血清中GIP抗体滴度水平,观察疫苗诱生抗体对GIP促进胰岛β细胞MIN6细胞分泌胰岛素的影响及两组在行为学方面存在的差异-结果:实验组首次免疫接种后4周,即产生了高滴度GIP特异性抗体,如期继续免疫接种,抗体水平进一步增高;实验组血清较对照组可以显著抑制GIP的促MIN6细胞胰岛素分泌作用(P < 0.01);行为学实验中,自发活动测试实验组总路程(P < 0.01)-平均速度(P < 0.01)-活动时间(P < 0.05)-活动次数(P < 0.01)均明显低于对照组,休息时间明显高于对照组(P < 0.05),有统计学差异,Morris水迷宫测试两组无明显统计学差异(P > 0.05)-结论:人工抗原GIP-KLH能打破机体免疫耐受,诱生GIP特异性抗体;GIP靶向的主动免疫干预治疗使大鼠自发活动性下降-

    Abstract:

    Objective:To investigate the effect of active immunization against gastric inhibitory popeptide(GIP)on behavior of SD rats. Methods:Male rats were fed with high-fat diet and divided into control group and GIP immunized group. Animals of experimental group and control group were immunized subcutaneously with 400 -滋g of GIP-KLH and KLH at the indicated time points respectively. GIP-specific antibody titers were determined by ELISA according to standard protocols differences on GIP-induced the insulin secretion of MIN6 cells using GIP-specific antibodies and behavior between two groups were investigated. Results:After four weeks of single immunization,high GIP-specific antibody titers were induced. Antibody levels further increased with continue injections. Compared with the control group,GIP-KLH immunized rats’serum containing specific antibodies significantly inhibited GIP-induced the insulin secretion on MIN6 cells(P < 0.01).Compared with control rats,distance(P < 0.01),speed(P < 0.01),active times(P < 0.05),the number of activities(P < 0.01)in spontaneous activity test were different(P < 0.05)significantly in GIP immunised group. Both the two groups showed no significant differences in test section of Morris water maze test(P > 0.05). Conclusion:Active immunization against GIP resulted in high,GIP-specific antibody titers. GIP immunized rats had a decreasing spontaneous activity.

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田建卿,王 越,林 宁,邹大进,孙树汉,曹 怡,王奇金.抑胃多肽主动免疫治疗对大鼠行为活动的影响[J].南京医科大学学报(自然科学版),2009,29(7):939-943

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  • 收稿日期:2009-02-26
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