环氧化酶-2单核苷酸多态性与结直肠癌生物学行为的相关性研究
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Single nucleotide polymorphism in cyclooxygenase-2 and its association with biological behavior of colorectal cancer
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    目的:探讨环氧化酶-2(COX-2)基因启动子区-1195G>A单核苷酸多态性与结直肠癌病理参数以及与COX-2表达的关系-方法:对118例结直肠癌血标本,应用SYBR GREEN PCR溶解曲线方法进行COX-2启动子区-1195G>A基因型分析-结果:含有A等位基因的GA 或AA基因型相对于GG基因型,淋巴结转移以及肿瘤分期差异显著(P=0.007及P=0.010)-GA-AA基因型COX-2阳性率相对于GG基因型OR分别为3.01(95%CI 1.07~8.46,P=0.036)-2.79(95%CI 1.17~6.64,P=0.020)-结论:COX-2-1195G>A多态性与淋巴结转移-肿瘤分期以及COX-2表达密切相关,该位点含A等位基因提示淋巴结转移-肿瘤分期晚高风险,COX-2免疫组化阳性率高-

    Abstract:

    Objective:To examin the single nucleotide polymorphism(SNP) in COX-2 promoter(-1195G>A) and the association between pathological parameters and COX-2 expression in colorectal cancer. Methods:Genotyping of the COX-2 promoter -1195G>A was carried out by single-tube SYBR GREEN PCR with Tm-shift primers. 118 samples of colorectal cancer patients were anaylzed by genotyping. Results:Stratified analysis indicated that an elevated risk of lymph node metastasis and advanced stage was observed in subjects who carried at least one A allete(AA or GA) compared with patients with GG genotype(P=0.007,0.010,respectively). High expression of COX-2 was found in subjects carrying AA or GA genotype compared with patients with GG genotype(OR= 3.01and 2.79 respectively). Conclusion:COX-2 SNP-1195G>A was associated with clinicobiological behaviors of tumor, such as lymph node metastasis,tumor stage, and expression of COX-2 protein. -1195A allele indicated high risk of lymph node metastasis,advanced stage and high rate of COX-2 staining positive in immunohistochemisty.

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陈志刚,魏 嘉,禹立霞,钱晓萍,刘宝瑞.环氧化酶-2单核苷酸多态性与结直肠癌生物学行为的相关性研究[J].南京医科大学学报(自然科学版),2009,29(7):963-966

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  • 收稿日期:2009-01-19
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