Objective:To investigate antibiotic resistance and carbapenemase genotype in Acinetobacter baumannii. Methods:Twenty-two strains of Acinetobacter baumannii resistant to carbapenem and 3 strains susceptible to carbapenem were collected. Their MIC were done by Kribry-Bauer methods and microbroth dilution. The genes were amplified by polymerase chain reaction(PCR), and the PCR products were sequenced. Results:The 22 strains were multi-drug resistance. OXA-23 gene was identified in 18 of the 22 strains, but the gene was not identified in 3 susceptible strains. OXA-24 was not identified in either resistant or susceptible strains. The sequenced results were absolutely homology with blaOXA-23 gene. Conclusion:Production of OXA-23 in Acinetobacter baumannii is one of the molecular mechanisms responsible for carbapenem resistance, and the code gene was blaOXA-23.