骨髓衰竭患者骨髓间充质干细胞形态和生长特征
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南京医科大学科技发展基金项目(07NMUM080);江苏省自然科学基金(BK2008236)


Morphology and growth characteristics of bone marrow mesenchymal stem cell from bone marrow failure syndromes patients
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    摘要:

    目的:观察和比较再生障碍性贫血(AA)-骨髓增生异常综合征(MDS)和正常人来源的骨髓间充质干细胞(MSCs)的形态和生长特征的差异-方法:从AA患者-MDS患者及正常人骨髓中分离MSCs,进行体外培养,观察细胞形态特征,绘制生长曲线描述细胞生长情况-结果:研究发现AA患者的骨髓MSCs的形态与正常对照组无异,均为梭形成纤维细胞样,但AA患者的骨髓MSCs生长缓慢,集落形成时间及细胞融合达90%所需时间均长于正常对照组,P3代细胞增殖能力低于正常对照组-MDS患者的骨髓MSCs的形态-集落形成时间及细胞融合达90%所需的时间与正常组无明显差别,P3代细胞增殖能力与正常正常对照组无异-结论:AA和MDS患者的骨髓MSCs的形态与正常对照组无异,但AA患者骨髓MSCs的生长速度和增殖能力明显低于正常对照组,而MDS患者骨髓MSCs的细胞形态-生长速度和细胞增殖能力与正常对照组相比无明显差别-

    Abstract:

    Objective:To investigate and compare the differences on morphology and growth characteristics of mesenchymal stem cells(MSCs)derived between bone marrow of aplastic anemia(AA)patients,myelodysplastic syndrome(MDS)patients and normal individuals. Methods:MSCs were isolated from bone marrow sample of AA patients,MDS patients and normal individuals and cultured in vitro. The morphology and growth curves feature were observed. Results:MSCs derived from AA patients and normal individuals were similar in cell morphology. Both were fibroblast-like spindle. But MSCs from AA patients grew slower than those of the normal control group. The time of colony-forming of the MSCs from AA patients was longer. And the time required for the MSCs from AA patients reaching 90% confluency was also longer than that of the normal control group. The proliferation capacity of MSCs from AA patients at passage 3 was weaker than that of normal individuals. While,MDS patients and normal individuals derived MSCs were similar in cell morphology as well as the time of cell colony-forming and cell reaching 90% confluency. There was no difference in the proliferative ability of MSCs at passage 3 between MDS patients and normal control group. Conclusion:The morphology of MSCs showed a similar characteristics among the three groups. But compared with MSCs from normal individuals,MSCs from AA showed significant slower growth rate and weaker proliferative ability. Whereas,there were no significant differences between MSCs from MDS and those of normal control group in morphology,growth rate and proliferation capacity.

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杨诗梅,陆世丰,陆 化,费小明,张建富,沈文怡,刘增艳,李建勇.骨髓衰竭患者骨髓间充质干细胞形态和生长特征[J].南京医科大学学报(自然科学版),2009,29(11):1498-1502

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  • 收稿日期:2009-05-18
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