SIRPα1转染对乳腺癌细胞株MCF-7增殖和凋亡的影响
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国家自然科学基金资助(30871019)


Effect of SIRPα1 on cell proliferation and apoptosis in human breast cancer cell line MCF-7
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    摘要:

    目的:探讨外源性SIRPα1基因对乳腺癌细胞株MCF-7增殖和凋亡的影响及相关的分子生物学机制-方法:乳腺癌细胞株MCF-7本身不表达SIRPα1,将SIRPα1基因转染入MCF-7,用EGF刺激,Western blot法检测JNK蛋白及磷酸化的JNK蛋白表达水平的变化,MTT法检测细胞的增殖活性,TUNEL法检测细胞凋亡水平-结果:转染SIRPα1基因后,JNK蛋白的总量没有发生变化,而磷酸化的JNK减少,同时细胞增殖受抑,凋亡增加-SIRPα1胞内段的酪氨酸残基可被多种有丝分裂原活化而发生磷酸化-用EGF刺激转染SIRPα1质粒的MCF-7细胞,与未加刺激的相比,细胞凋亡无明显变化-结论:SIRPα1能促进乳腺癌细胞凋亡,抑制细胞增殖,是一种候选的抑癌基因-

    Abstract:

    Objective:To investigate the effect of exogenous SIRPα1 gene on the proliferation and apoptosis of MCF-7 and explore the molecular biology mechanism. Methods:SIRPα1 gene was transfected into SIRPα1-negative MCF-7 with lipofectamine. Cell were stimulated by EGF and the expression of JNK and pho-JNK were examined by Western blot. The proliferation activity was assessed by MTT and the apoptosis of MCF-7 was detected by TUNEL. Results:After SIRPα1 was transfected into MCF-7,there was no change of JNK protein expression but a remarkable decrease of p-JNK. In addition,the expression of SIRPα1 also reduced MCF-7 cell vitality and induced cell apoptosis. The intracellular part of SIRPα1 can be phosphorylated in response to a variety of growth factors. But there was no significant change of cell apoptosis after EGF stimulated MCF-7 which were transfected with exogenous SIRPα1 gene. Conclusion:SIRPα1 can induce cell apoptosis,inhibit cell proliferation in MCF-7 cell line and serve as a potential anti-oncogene.

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张璐瑶,王〓晨,曾 科. SIRPα1转染对乳腺癌细胞株MCF-7增殖和凋亡的影响[J].南京医科大学学报(自然科学版),2009,29(11):1503-1507

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  • 收稿日期:2009-05-11
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