超声微泡造影剂介导血管生成素-1基因治疗下肢缺血
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Microbubble mediating Ang-1 gene therapy of lower limb ischemia by using ultrasound
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    目的:探讨用超声微泡造影剂介导血管生成素-1(Ang-1)基因治疗兔下肢缺血的可行性-方法:将15只新西兰大白兔左侧股动脉及其分支切断结扎后随机分成3组:A组采用局部注射pEGFP/Ang-1质粒与超声微泡造影剂的混合物并用超声照射;B组采用局部注射pEGFP/Ang-1质粒;C组注射生理盐水作为对照组-治疗4周后进行血管造影,了解新生血管情况,免疫组化方法检测Ang-1的蛋白表达-结果:在侧支循环建立-胞浆棕褐色程度-新生血管方面,A组较多,B组其次,C组较少-结论:用超声微泡介导的Ang-1基因转染,可促进缺血骨骼肌的血管新生和侧支循环建立,为下肢缺血性疾病的基因治疗提供了一种新的途径-

    Abstract:

    Objective:To investigate the feasibility of microbubble mediating Ang-1 gene therapy of lower limb ischemia by using ultrasound. Methods:Fifteen rabbits with the left femoral artery ligation were randomly and averagely devidied into 3 groups:group A was locally intramuscular injected with the mixture of plasmid pEGFP/Ang-1 and microbubble and accepted ultrasound local radiation,group B was locally intramuscular injected with the plasmid pEGFP/Ang-1,and group C was injected with NS. Four weeks post-treatment,angiography was applied to evaluate the collateral circulation,and the expression of Ang-1 was evaluate by immunochemical staining. Results:In the matter of the collateral circulation,the degree of brown in cytoplasm,new vascular of the treatment region,group A was superior to the group B,the group B was superior to the group C. Conclusion:Microbubble mediating Ang-1 gene therapy by using ultrasound could promote the collateral circulation and new vessels,it may be a new methods for the treatment of limb ischemia diseases.

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陶〓政,徐三荣,刘文新,陈保定.超声微泡造影剂介导血管生成素-1基因治疗下肢缺血[J].南京医科大学学报(自然科学版),2009,29(11):1534-1537

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  • 收稿日期:2009-05-12
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