川芎嗪对新生鼠缺血缺氧脑损伤HIF-1α表达的影响
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The effect of liguistrazine on the HIF-1ɑ expression in neonatal rats with hypoxic ischemic brain damage
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    目的:探讨实验性大鼠缺血缺氧脑损伤(HIBD)时低氧诱导因子-1α(HIF-1α)的表达及川芎嗪对HIF-1ɑ表达的影响-方法:新生SD大鼠结扎左侧颈总动脉并置于缺氧环境中,制备HIBD模型-川芎嗪干预组在缺血前和缺血后腹腔注射川芎嗪(50 mg/kg)-应用逆转录PCR和免疫组化方法观察川芎嗪对HIBD新生鼠皮质部位HIF-1α mRNA 及蛋白表达的影响-结果:缺血缺氧2 h后HIF-1α mRNA 及蛋白表达有增加的趋势,但与假手术组相比无统计学意义(P > 0.05);川芎嗪干预组HIF-1α mRNA 及蛋白表达显著增加,明显高于缺血缺氧组(P < 0.05)-结论:川芎嗪促进新生鼠缺血缺氧脑损伤皮质部位HIF-1α mRNA 及蛋白表达,是其神经保护作用的重要机制-

    Abstract:

    Objective:To explore the effect of liguistrazine on the expression of hypoxia-induced factor-1α(HIF-1α)in neonatal rats with hypoxic-ischemic brain damage(HIBD). Methods:Models of 7-day-old neonatal rats with HIBD were established by occluding the left common carotid artery and subjected to inhalation of 8% oxygen. The mice of treated group were injected with liguistrazine intraperitoneally(50 mg/kg) before and after the operation. The expression of HIF-1α in cortex was measured by RT-PCR and immunohistochemical staining. Results:Although the levels of HIF-1α mRNA and protein expression at 2 h after HIBD were higher than those of sham group,there was no significance(P > 0.05) .While the expression of HIF-1α mRNA and protein were remarkably higher in liguistrazine treated group than those of HIBD group(P < 0.05). Conclusion:Liguistrazine can increase the expression of HIF-ɑ mRNA and protein in neonatal rats with HIBD,which may be the molecular mechanism of its neuroprotective effect.

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李〓娟,俞海国,陆亚东.川芎嗪对新生鼠缺血缺氧脑损伤HIF-1α表达的影响[J].南京医科大学学报(自然科学版),2009,29(11):1542-1544

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  • 收稿日期:2009-04-13
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