体外循环期间细胞因子TNF-α?IL-6与中性粒细胞的变化及其相互影响
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:


Changes of neutrophils,cytokines TNF-α,IL-6 during cardiopulmonary bypass
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:观察体外循环(CPB)炎症反应过程中致炎性细胞因子TNF-α-IL-6 与中性粒细胞的变化规律及其相关性-方法:选择30例体外循环下行瓣膜置换术的患者,分别于CPB前-CPB 40 min-CPB停机和停机后2-6-24 h以酶联免疫法(ELISA)测定血清TNF-α-IL-6 浓度,全血细胞分析仪测定外周血中性粒细胞数量-结果:CPB后各个时间点的血清TNF-α-IL-6浓度和中性粒细胞值较CPB前明显升高,差异有显著性(P < 0.01)-血清TNF-α-IL-6水平在CPB开始后,逐渐升高-CPB结束后2 h,TNF-α的血清水平达到高峰,CPB结束后24 h TNF-α仍维持在很高的水平-血清IL-6水平在 CPB结束后6 h达到高峰,然后下降,CPB结束后24 h仍维持在较高水平-中性粒细胞的变化趋势同TNF-α-2组TNF-α-IL-6血清水平(高峰值)与CPB时间及主动脉阻断(ACC)时间正相关(P < 0.01)-围手术期中性粒细胞值与TNF-α-IL-6血清水平有一定的相关性-结论:CPB可触发全身炎症反应,表现为外周血中性粒细胞数量升高与血清TNF-α-IL-6水平升高;CPB和ACC的时间长短影响TNF-α-IL-6的释放,可能影响到术后患者炎性反应的程度;CPB期间血清TNF-α水平与血清IL-6水平-中性粒细胞的升高相关-

    Abstract:

    Objective:To study the relationship of the changes of amount of neutrophils to concentration of IL-6,TNF-α during cardiopulmonary bypass. Methods:The serum levels of TNF-α,IL-6 were measured by Enzyme Linked Immunosorbent Assay(ELISA) method in patients undergoing heart valve replacement,and the amount of neutrophils was also measured. Serial blood samples were collected at the following intervals respectively:before CPB,40 min after CPB and 0 h,2 h,6 h,24 h after the end of CPB. Results:The serum levels of TNF-α,IL-6 and the amount of neutrophils after CPB were significantly higher than that before CPB(P < 0.01).The serum levels of TNF-α and IL-6 gradually increased after the beginning of CPB. The serum level of TNF-α reached the peak at 2 h after the end of CPB,and that still maintained a high level at 24 h after the end of CPB. The amount of neutrophils had the similar change. The serum level of IL-6 reached the peak at 6h after the end of CPB,then began to decrease but still higher than the concentration before CPB significantly. The concentration of TNF-α and IL-6 was correlated positively to the time of CPB and ACC(P < 0.01). There were correlations between the amount of neutrophils and the concentrations of TNF-α and IL-6. Conclusion:Cardiac surgery with cardiopulmonary bypass (CPB) leads to a systemic inflammatory response syndrome(SIRS) with secretion of cytokines and neutrophils. The time of CPB and ACC influences release of TNF-α and IL-6,so it may change the degree of SIRS in patients after operation. There are correlations between the amount of neutrophils and the concentrations of TNF-α and IL-6 during CPB.

    参考文献
    相似文献
    引证文献
引用本文

杨晓刚,郭德和,边〓虹,何进喜.体外循环期间细胞因子TNF-α?IL-6与中性粒细胞的变化及其相互影响[J].南京医科大学学报(自然科学版),2009,29(11):1585-

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2009-04-12
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明