黄芪微乳载入修饰后胶原对人脐静脉内皮细胞增殖的影响
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国家自然科学基金资助(30572332)


Effects of Astragalus mongholicus microemulsion modified-collagen on proliferation of human umbilical vascular endothelial cells in vitro
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    目的:研究黄芪微乳载入修饰后胶原对人脐静脉内皮细胞(HUVEC)增殖的影响,进一步探讨黄芪微乳对HUVEC增生的作用及其机制-方法:体外培养HUVEC细胞,用含1%FCS的RPMI-1640培养液同步24 h后,分为2组进行观察:空白微乳胶原组和黄芪微乳胶原组,并设定空白微乳和黄芪微乳的浓度分别为16.90-8.50-4.20-2.10-1.05 μg/ml等5个浓度,每组每个浓度设3个复孔-干预48 h后,采用MTT法和流式细胞术检测各组细胞的增殖情况;实时荧光定量RT-PCR检测细胞内血管内皮细胞生长因子(VEGF)mRNA表达-结果:干预48 h后,在浓度分别为16.9-8.5-4.2 μg/ml的黄芪微乳胶原组HUVEC的吸光度值-细胞分裂增殖指数和VEGF mRNA表达均明显高于相应浓度的空白微乳胶原组(P < 0.05,P < 0.01)-结论:黄芪微乳能促进HUVEC的增殖,并上调HUVEC的VEGF mRNA表达,推测黄芪微乳可能具有促进血管生成的作用-

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    Objective:To investigate the effect of Astragalus mongholicus microemulsion modified-collagen on proliferation of human umbilical vascular endothelial cells(HUVEC) in vitro. Methods:Cell line of HUVEC was cultured and all experiments were performed using the cells at the third passage. After synchronization of cells growth,HUVEC was divided into blank microemulsion modified-collagen group and Astragalus mongholicus microemulsion modified-collagen group. Each group was exposed to the concentrations of 16.90,8.50,4.20,2.10 and 1.05 μg/ml,respectively. The proliferation of HUVEC was measured by MTT and flow cytometry assay. The real-time quantitative PCR was used to detect the expression of vascular endothelial cell growth factor(VEGF) mRNA in HUVEC. Results:After co-culturing for 48 h,the proliferation and VEGF mRNA expression of HUVEC in Astragalus mongholicus microemulsion modified-collagen group at the concentrations of 16.9,8.5 and 4.2 μg/ml significantly increased compared with the groups on the same concentrations in blank microemulsion modified-collagen control(P < 0.05,P < 0.01). Conclusion:Astragalus mongholicus microemulsion not only promotes the proliferation of HUVEC,but also upregulates the expression of VEGF mRNA. This study demonstrateds that Astragalus mongholicus microemulsion may promote angei-anagenesis.

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蔡旭兵,潘立群.黄芪微乳载入修饰后胶原对人脐静脉内皮细胞增殖的影响[J].南京医科大学学报(自然科学版),2009,29(12):1653-

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  • 收稿日期:2009-06-19
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