丹酚酸B对TGF-β1诱导的人肾小管细胞转分化的影响
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

江苏省高校自然科学基础研究项目(08KJB320006);江苏省常州市社会发展项目(CS2006217)


Prevent effects of salvianolic acid B on TGF-β1-induced epithelial mesenchymal transition in HK2 cells
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探讨丹酚酸B对转化生长因子(TGF)-β1诱导的肾小管上皮细胞—间充质细胞转分化的影响-方法:将体外培养的人近端肾小管上皮细胞系(HK-2)细胞分为3组:①对照组:未加入丹酚酸B或者TGF-β1;②TGF-β1组:在细胞培养基中加入TGF-β1(浓度为5 ng/ml);③丹酚酸B+TGF-β1组:在细胞培养基中分别加入丹酚酸B和TGF-β1(浓度为5 ng/ml),按丹酚酸B的浓度分为A-B-C-D 4个亚组:丹酚酸B的浓度分别为A组:0.1 μmol/L,B组:1 μmol/L,C组:10 μmol/L,D组:100 μmol/L-72 h后,采用倒置相差显微镜观察细胞形态学变化;采用逆转录聚合酶链反应(RT-PCR)和免疫细胞化学染色检测细胞α-平滑肌肌动蛋白(α-SMA)和E-cadherin的表达情况-结果:TGF-β1组HK-2细胞从原有典型的上皮细胞形态转变为长梭形肌成纤维细胞形态;胞浆内大量表达α-SMA,同时E-cadherind的表达明显减少;不同剂量丹酚酸B治疗可减轻TGF-β1诱导的细胞形态学改变和胞浆内α-SMA的表达,同时E-cadherind的表达得到明显恢复,且呈剂量依赖性-结论:丹酚酸B具有阻止慢性肾脏疾病进行性发展的潜能,而这一作用与其能有效阻止TGF-β1诱导的HK-2细胞转分化有关-

    Abstract:

    Objective:To investigate the protective effects of salvianolic acid-B (Sal B) on the trans-differentiation of renal tubular epithelial cells and to elucidate the mechanism of Sal B on renal fibrosis. Methods:Human kidney proximal tubular cell line (HK-2) was used as the proximal tubular cell model. Cells were divided into six groups as follows:control group,transforming growth factor-β1 (TGF-β1) (5 ng/ml) group,TGF-β1 (5 ng/ml) plus Sal B (0.1 μmol/L,1 μmol/L,10 μmol/L,and 100 μmol/L) groups. Epithelialto-mesenchymal transition(EMT) was induced with 5 ng/mL of human TGF-β1. The effect of Sal B on cell morphology was observed by phase contrast microscopy,and the expressions of α-smooth muscle actin(α-SMA) and E-cadherin were measured by immunocytochemistry and RT-PCR. Results:Our results revealed that Sal B not only prevented the expression of α-SMA but also prohibited the decrease of the epithelial marker E-cadherin in HK-2 cells in a dose-dependent manner. Simultaneous incubation of Sal B with TGF-β1 could protect the change to the myofibroblast phenotype and restored the epithelial morphology of the HK-2 cells. Conclusion:These observations strongly suggest that Sal B is a potent inhibitor of TGF-β1-induced EMT and may be a promising agent for treating tubulointerstitial fibrosis.

    参考文献
    相似文献
    引证文献
引用本文

芮国华,潘荣华,姚〓刚,武晓春,徐玲玲.丹酚酸B对TGF-β1诱导的人肾小管细胞转分化的影响[J].南京医科大学学报(自然科学版),2009,29(12):1685-1689

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2009-05-19
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明