鼠源性单克隆抗体Met4用于检测胃癌组织Met的表达及临床意义
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南京医科大学科技发展基金项目(06NMUM009);横向合作项目(Van Andel Institute)(ky1011711084000092)


Mouse monoclonal antibody Met4 for detecting Met expression of human gastric cancer and clinical significance
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    目的:观察研究肝细胞生长因子受体Met在胃癌组织及相对应的淋巴结转移灶中的表达水平,同时比较分析抗Met单克隆抗体Met4和多克隆抗体C28在检测Met表达水平中的差别-方法:采用免疫组织化学间接法检测胃癌组织芯片中Met表达水平,比较Met4与C28的检测效能-结果:Met在细胞膜及胞浆阳性表达率在不同病理分级组间无统计学意义(P > 0.05)-Met4组Met的阳性染色部位于细胞膜(浆),而C28组Met阳性染色部位在细胞浆中,二者在Met亚细胞染色定位上有差别-Met4的阳性检出率(58.3%)高于C28阳性检出率(30.5%),具有统计学意义(P < 0.05)-Met4组21例胃癌原发灶Met阳性病例中14例淋巴结转移灶Met表达阳性,C28组11例原发灶Met阳性病例中6例淋巴结转移灶Met表达阳性-原发灶中Met阳性表达与转移淋巴结中Met的阳性表达有显著相关性(P < 0.05)-结论:Met阳性表达可能在肿瘤的淋巴转移过程起重要作用-Met4用于肿瘤的诊断及靶向治疗,具有良好的临床应用前景-

    Abstract:

    Objective:To investigate the expression level of Met in human gastric cancer,and compare the effects of mouse anti-human Met monoclonal antibody Met4 with that of commercial rabbit anti-human Met polyclonal antibody C28(sc-161,Santa Cruz,USA)on detection of the formalin-treated Met protein in tissue microarrays(TMA) of human gastric cancer. Methods:Standard immunohistochemistry (IHC) method of Met4 and C28 was used to analyze the expression of Met in TMA of gastric cancer. The reactivity efficacies of Met4 and the polyclonal antibody C28 were compared. Results:The expression of Met in membrane on cytoplosm had no significant difference among various pathological stages of cancer(P > 0.05). Met exhibited a combined membranous and (or) cytoplasmic pattern stained by Met4,while analysied by C28,Met staining revealed positive signals in cytoplasm of tumor cells. The subcellular localizations of Met were disclosed differently by these two antibodies. The positive expression rate of Met detected by Met4 was significantly higher than that by C28(P < 0.05). In Met4-staining group,14 of 21 cases with Met positive expression in primary tumor exhibited Met positive expression in its lymph metastases simultaneously. The similar result was displayed in C28 group. The elevated expression of Met in primary gastric cancer may predict tumor lymphatic metastatic potential. Conclusion:Overexpression of Met in primary gastric cancer may be associated with lymphatic metastasis of tumor. Met4 can work as anuseful reagent for tumor diagnosis and targeted therapy.

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黄〓嫣,郭振英,彭〓韬,张爱霞,李芸茜,曹伯良.鼠源性单克隆抗体Met4用于检测胃癌组织Met的表达及临床意义[J].南京医科大学学报(自然科学版),2010,(1):7-11

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  • 收稿日期:2009-08-12
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