Objective:To investigate the expression of survivin,cyclin D1 in human colorectal carcinoma and their relationship with clinicopathological features. Methods:The mRNA expression of survivin and cyclin D1 was determined by real-time quantitative PCR in 43 colorectal carcinoma tissues and para-carcinoma normal tissues. Subsequently,Western blot was used to quantitatively analyze the protein levels in colorectal carcinoma tissues and para-carcinoma normal tissues,respectively. Results:Real-time PCR and Western blot analysis revealed that survivin,cyclin D1 expression were also higher in colorectal carcinoma tissues than those in the paraneoplastic colorectal tissues(P < 0.01). There were no significant correlation between the copies of survivin,cyclin D1 and gender,age,tumor location,tumor size and depth of invasion(P > 0.05),but related to TNM stage and lymph node metastases,significantly(P < 0.05). Conclusion:Based on these findings,overexpression of survivin and cyclin D1 in the colorectal carcinoma suggested they may be participating in the genesis and development of colorectal carcinoma. Survivin and cyclin D1 could be useful tumor markers and prognostic factors for colorectal carcinoma.