产前单次应用地塞米松和氨溴索对胎鼠及子代鼠肺形态的影响
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江苏省卫生厅资助项目(H200642)


Effects of Prenatal Single-dose Administration of Dexamethasone and Ambroxol on Pulmonary Morphology of Fetal and Neonate Rats
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    摘要:

    目的:探讨产前单次联合给药小剂量地塞米松和氨溴索对胎鼠及生后子代大鼠肺形态发育的影响-方法:36只孕鼠随机分成2组:地塞米松加氨溴索干预组(n=9)和生理盐水对照组(n=9)-在妊娠第17天,干预组腹腔注射地塞米松0.2 mg/(kg·d)加氨溴索100 mg/(kg·d),对照组腹腔注射等体积的生理盐水-取两组孕19天,生后3天,生后7天胎(仔)鼠肺组织,通过光镜和电镜技术,观察在大鼠肺发育的不同阶段两组肺组织形态发育差异-结果:①光镜下:孕19天干预组胎鼠肺每视野平均原始肺泡计数大于对照组(P < 0.05),平均原始肺泡腔面积大于对照组(P < 0.01),平均原始肺泡间隔厚度小于对照组(P < 0.01)-生后3天干预组仔鼠肺每视野平均囊泡表面积大于对照组(P < 0.05),平均囊泡间隔厚度小于对照组(P < 0.05),平均囊泡计数与对照组相比无显著差异(P > 0.05)-生后7天干预组仔鼠肺每视野平均肺泡表面积大于对照组(P < 0.05),平均肺泡间隔厚度小于对照组(P < 0.05),平均肺泡计数与对照组相比无显著差异(P > 0.05);②透射电镜观察:孕19天胎鼠肺干预组Ⅱ型肺泡上皮细胞绒毛发育较对照组密集完整,可见板层小体,对照组难见板层小体-生后3天及7天的仔鼠肺干预组和对照组均可见大量板层小体,但干预组较对照组板层小体染色深-致密,且可见大量线粒体等发达的细胞器-结论:小剂量地塞米松联合氨溴索产前单次用药,不仅能促进大鼠胎肺成熟,也可促进其生后一段时期的肺发育且未产生明显不良影响-

    Abstract:

    Objective:To investigate effects of prenatal single-dose administration of dexamethasone and ambroxol on pulmonary morphology of fetal and neonate rats. Methods:Thirty-six pregnant rats were randomly assigned to two groups:the treatment group (n=9) and the control group (n=9). On day 17 of pregnancy,the rats in the treatment group were injected intraperitoneally with dexamethasone and ambroxol,while the rats in the control group were injected intraperitoneally with equivalent volumes of isotonic saline. The histologic structures of lungs of each group were observed with light microscope and trans-mission electron microscope on day 19 of pregnancy,postnatal days 3 and 7,respectively. The differences of pulmonary morphogenesis between two groups on different periods were studied. Results:(1) Under the light microscope:lungs in the treatment group had more alveolar numbers (P < 0.05),larger alveolar space (P < 0.01),and thinner alveolar septum (P < 0.01) compared with the control group on day 19 of pregnancy;lungs in the treatment group had larger alveolar space (P < 0.05),thinner alveolar septum (P < 0.05),while alveolar numbers had no significant difference (P > 0.05) compared with the control group on postnatal day 3;lungs in the treatment group had larger alveolar space (P < 0.05),thinner alveolar septum (P < 0.05),while alveolar numbers had no significant difference (P > 0.05) compared with the control group on postnatal day 7;(2) Under the electronic microscope:villi of type Ⅱ alveolar epithelial cells in the treatment group were denser and more integral than those in the control group;the lamellar body could be seen in the treatment group while hardly in the control group on day 19 of pregnancy. There were many lamellar bodies in both groups,while lamellar bodies in the treatment group were more anachromasis and condensed compared with the control group on the postnatal 3 days and 7 days. There were also many cellular organs such as bioblast in endochylema of the treatment group on the postnatal 3 days and 7 days. Conclusion:Prenatal single-dose administration of dexamethasone and ambroxol can promote lung maturation not only of fetal rats but also of neonate rats,without obvious harmful effects.

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浦潇丹,阚〓清,缪晓林,吴〓越,周晓玉.产前单次应用地塞米松和氨溴索对胎鼠及子代鼠肺形态的影响[J].南京医科大学学报(自然科学版),2010,(1):25-29111

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  • 收稿日期:2009-08-18
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