孕鼠维生素D缺乏对子鼠骨发育相关基因BMP-2?Cbfal?Dmp1表达的影响
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南京市医学科技发展一般性项目(ZKM06045);南京医科大学科技发展基金重点项目(06NMUZ024)


Effects of vitamin D deficiency during pregnancy on expression of bone development-related genes of offspring
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    目的:研究大鼠孕期维生素D缺乏对其子鼠骨发育相关基因BMP-2-Cbfa1-Dmp1表达的影响-方法:成熟雌性SD大鼠36只随机分为模型组和对照组各18只,模型组以不含维生素D的大鼠饲料避光喂养,对照组以标准饲料正常光照喂养-两周后与成熟SD雄性大鼠交配,分别取孕20天(E20 d)-生后1天(B1 d)和生后7天(B7 d) 子鼠左侧股骨-通过Real-TimePCR方法分别比较模型组和对照组胚胎和新生鼠长骨中BMP-2-Cbfa1-Dmp1基因在3个时间点表达的差异-结果:BMP-2 mRNA-Cbfa1 mRNA 及Dmp1 mRNA在E20 d-B1 d和B7 d的表达,模型组均明显低于对照组,差异有统计学意义(P < 0.05) -结论:BMP-2-Cbfa1-Dmp1均为骨发育相关基因,孕鼠维生素D缺乏可下调子鼠长骨中BMP-2-Cbfa1-Dmp1的表达水平,从而可能影响子鼠骨骼的发育-

    Abstract:

    Objective:To study the expression of BMP-2,Cbfa1 and Dmp1 on the femurs of offspring,whose mother is deficient of vitamin D during pregwanoy. Methods:36 adult female SD rats were chosen and allocated randomly into control group(18 rats) and model group (18 rats). The control group received vitamin D fodder with normal solarization and the model group received non-vitamin D fodder without solarization for 2 weeks. Then the female rats in two groups copulated with normal male rats. The left femurs were collected from the 20-day embryos(E20 d),the 1-day(B1 d) and 7-day(B7 d) newborns respectively. The expressions of bone development-related genes BMP-2,Cbfa1 and Dmp1 were measured by Real-Time PCR. Results:The expressions of BMP-2,Cbfa1 and Dmp1 mRNA in the model group were lower than those of the control group on E20d,B1d and B7d,respective (P < 0.05). Conclusion:The changes of expressions of bone development-related genes BMP-2,Cbfa1 and Dmp1 on the femurs of offspring are due to vitamin D deficiency of mother during the pregnancy,which would affect the offspring’s bone development.

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吴〓越,李婵娟,周晓玉,刘晓梅,张〓翔.孕鼠维生素D缺乏对子鼠骨发育相关基因BMP-2?Cbfal?Dmp1表达的影响[J].南京医科大学学报(自然科学版),2010,(1):30-

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  • 收稿日期:2009-06-08
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