ICI118551和福莫特罗对大鼠成骨样细胞增殖和凋亡的影响
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金面上项目(30600626)


Effects of ICI118551 and Formoterol on proliferation and apoptosis in rat osteoblast-like cells
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:研究选择性β2肾上腺素能受体激动剂福莫特罗(Formoterol)和阻滞剂ICI118551对体外由大鼠骨髓间充质干细胞(MMSCs)诱导分化的成骨样细胞增殖和凋亡的影响,探讨β2肾上腺素能受体信号对骨代谢的影响-方法:取3周龄雌性SD大鼠,全贴壁筛选法培养MMSC,用条件培养液诱导向成骨细胞分化后分别加入不同浓度Formoterol和ICI118551,检测细胞增殖率和细胞凋亡率-结果:MMSC成功诱导分化为成骨样细胞,不同浓度(10-5~10-9 mmol/L)的Formoterol均可抑制成骨样细胞增殖-高浓度ICI118551(10-5和10-6 mmol/L)抑制成骨样细胞细胞增殖,低浓度ICI118551(10-8和10-9 mmol/L)则可促进成骨样细胞增殖-不同浓度(10-5~10-9 mol/L)的福莫特罗和ICI118551对成骨样细胞的凋亡无明显影响-结论:β2肾上腺素能受体激动剂可抑制体外大鼠成骨样细胞增殖,而阻滞剂对成骨样细胞增殖的影响与其浓度有关-β2肾上腺素能受体激动剂和阻滞剂对大鼠成骨样细胞凋亡无明显影响-

    Abstract:

    Objective:To evaluate the effects of Formoterol(β2-adrenergic receptor-specific agonist)and ICI118551(β2-adrenergic receptor-specific antagonist) on proliferation and apoptosis of rat osteoblast-like cells induced from marrow-derived mesenchymal stem cells(MMSCs). Methods:MMSCs by whole bone marrow adherent culture derived from a three-week-old female SD rat are induced to osteoblast-like cells .After different concentrations of Formoterol and ICI118551 are added into the culture medium,The cell proliferation is evaluated with the method of MTT and the cell apoptosis is evaluated by the methods of Annexin V-FITC/PI and TUNEL. Results:The proliferation of cells cultured with different concentrations(10-5~10-9 mmol/L) of Formoterol are all obviously low. As to ICI118551,high concentrations (10-5 and 10-6 mol/L)can inhibit the proliferation of cells while low concentrations(10-8 and 10-9 mol/L) can enhance proliferation. Both Formoterol and ICI118551 have minimal effects on the cell apoptosis. Conclusion:The β2-adrenergic receptor-specific agonist can inhibit the proliferation of rat osteoblast-like cells in vitro while the effects of theβ2-adrenergic receptor-specific antagonist are not single,depending on its concentration. Both of them have minimal effects on the cell apoptosis.

    参考文献
    相似文献
    引证文献
引用本文

胡安全,余利鹏,韦永中,程庆华. ICI118551和福莫特罗对大鼠成骨样细胞增殖和凋亡的影响[J].南京医科大学学报(自然科学版),2010,(2):161-165

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明