PGE2通过cAMP-PKA信号转导通路促进肝癌细胞VEGF表达
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金资助项目(30470784,30871015)


Prostaglandin E2 increased the expression of vascular endothelial growth factor (VEGF) by activation of cAMP-PKA pathway in hepatocellular carcinoma cells
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探讨PGE2对人肝癌细胞HuH7 VEGF表达的影响及其可能涉及的信号转导通路-方法:用PGE2-EP1-4四种受体激动剂-SQ22536+PGE2-H89+PGE2处理HuH7细胞,Real-time PCR-Western blot-ELISA等检测VEGFmRNA-蛋白表达的变化-结果:10 μmol/L PGE2处理HuH7细胞24 h后,VEGFmRNA的表达水平上升了264%(P < 0.01)-细胞培养上清中VEGF蛋白表达水平上升了24%(P < 0.01);10 μmol/L PGE2处理HuH7细胞6-12-24 h后,胞浆中VEGF蛋白的表达水平分别上升了13.6%-25.7%-39.6% (P < 0.01);10 μmol/L EP2受体激动剂处理HuH7细胞24 h后,胞浆-细胞培养上清中VEGF蛋白表达水平与对照组相比分别上升了32.3%-20.1%(P < 0.01),而10 μmol/L EP1-EP3-EP4受体激动剂处理HuH7细胞24 h后,VEGF的表达水平无明显改变;30 μmol/L PKA抑制剂(H89)-500 μmol/L腺苷酸环化酶抑制剂(SQ22536)处理HuH7细胞24 h后,胞浆VEGF的表达水平分别为对照组的91.6%-89.6%,细胞培养上清中VEGF的表达水平分别为对照组的98.3%-91.9%,与PGE2组相比均有统计学差异(P < 0.01)-结论:PGE2可上调HuH7细胞VEGF的表达,其作用机制可能是通过EP2受体激活cAMP-PKA信号转导通路所致-

    Abstract:

    Objective:To explore VEGF expression induced by PGE2 in human hepatocellular carcinoma cells HuH7 and the possible mechanisms of signal transduction pathways. Methods:HuH7 cells were treated with PGE2,EP1-4 receptor agonist,SQ22536+PGE2,H89+PGE2. VEGF mRNA and protein expression were examined by Real-time PCR,Western blot and ELISA in HuH7 cells. Results:The expression of VEGF mRNA in HuH7 cells were increased by 264%(P < 0.01),and the expression of VEGF in culture supernatant of HuH7 cells were increased by 24%(P < 0.01)after treated with PGE2(10 μmol/L) for 24 h. The expression of VEGF in cytoplasm of HuH7 cells were increased by 13.6%,25.7%,39.6% (P < 0.01)after treated with PGE2 (10 μmol/L) for 6 h,12 h,24 h respectively. The expression of VEGF in cytoplasm and culture supernatant of HuH7 cells were increased by 32.3% and 20.1%(P < 0.01)after treated with EP2 receptor agonist(10 μmol/L) for 24 h respectively,but have no changes after treated with EP1,EP3,EP4(10 μmol/L) receptor agonist. After the treatment of H89 (30 μmol/L) and SQ22536(500 μmol/L) for 24 h,the expression of VEGF in cytoplasm of HuH7 cells was decreased by 91.6% and 89.6%,and the expression of VEGF in culture supernatant of HuH7 cells was decreased by 98.3% and 91.9%,respectively,vs PGE2(P < 0.01). Conclusion:It is suggested that PGE2 might up-regulate the expression of VEGF through EP2 receptor of HuH7 cells,which could be partly related to the cAMP-PKA signal conduction pathway.

    参考文献
    相似文献
    引证文献
引用本文

王耀辉,马〓娟,束〓为,彭〓韬,张〓海,白小明,张〓丽,冷〓静. PGE2通过cAMP-PKA信号转导通路促进肝癌细胞VEGF表达[J].南京医科大学学报(自然科学版),2010,(3):299-303

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2009-10-13
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明