脱垂子宫主骶韧带ERRα表达及17-β雌二醇对其成纤维细胞周期影响的研究
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2006年江苏省社会发展科技计划指导性项目(BS2006507)


A study on ERRα expression in prolapsed uterine cardinal ligaments and the effect of 17-β estradiol to fibroblast’s cell cycle
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    摘要:

    目的:探讨ERRα在脱垂子宫主骶韧带的表达及17-β雌二醇(17-βE2)对其成纤维细胞周期影响-方法:取10例未脱垂子宫主骶韧带组织和10例脱垂子宫主骶韧带组织,使用流式细胞仪定量检测ERRα表达情况-对10例脱垂子宫主骶韧带组织进行原代细胞培养,传代细胞分别用浓度0.1-1.0-10.0-100.0 nmol/L 17-βE2作用48 h,流式细胞仪术分析刺激后细胞的细胞周期,采用增殖指数(PI)进行比较-结果:ERRα在子宫主骶韧带有表达,且未脱垂子宫主骶韧带ERRα表达量高于脱垂子宫主骶韧带的表达量(P < 0.05)-10.0 nmol/L的17-βE2作用组的PI高于对照组(P < 0.05)-而100.0 nmol/L的17-βE2作用组的PI低于对照组(P < 0.05)-结论:脱垂子宫主骶韧带ERRα表达量降低-10.0 nmol/L 17-βE2能促进韧带成纤维细胞生长,100.0 nmol/L 17-βE2能抑制韧带成纤维细胞生长-

    Abstract:

    Objective:To explore the ERRα expression in cardinal ligament and the effect of 17-β estradiol(17-βE2) on the proliferation of fibroblasts derived from cardinal ligament in patients with uterine prolapse. Methods:The tissues of cardinal ligament were collected from 10 patients with uterine prolapse and 10 normal controls for the detection of ERRα expression by flow cytometry;total of 10 cell strains of cardinal ligament fibroblasts derived from patients with uterine prolapse were established by primary cell culture and were treated with different concentrations of 17-βE2 (0 nmol/L,0.1 nmol/L,1.0 nmol/L,10.0 nmol/L,100.0 nmol/L) for 48 h. The effect of E2 on cell proliferation was then measured by the proliferous index(PI). Results:① ERRα were expressed in cardinal ligament of patients with uterine prolapse and normal controls,and the ERRα expression level of cardinal ligament was higher in normal controls than that in patients with uterine prolapse(P < 0.05). ② The PI of fibroblasts at 10.0 nmol/L 17-βE2 concentration was higher than that of control. The PI of fibroblasts at 100.0 nmol/L 17-βE2 concentration was lower than that of control. Conclusion:①The ERRα expression level of cardinal ligament decreased in patients with uterine prolapse. ② The effect of different concentrations of 17-βE2 on the proliferation of fibroblasts derived from cardinal ligament in patients with uterine prolapse was different:17-βE2 promoted fibroblasts proliferation at concentration of 10.0 nmol/L,but it induced apoptosis of the cells at 100.0 nmol/L concentration.

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邹少晗,龚〓健,王家俊,陈艳琴.脱垂子宫主骶韧带ERRα表达及17-β雌二醇对其成纤维细胞周期影响的研究[J].南京医科大学学报(自然科学版),2010,(3):339-344

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