脂多糖诱导肥大细胞释放HMGB1研究
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江苏省自然科学基金资助(BK2006027)


Extracellular release of HMGB1 in lipopolysaccharide-induced mast cells and related signaling pathways
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    摘要:

    目的:研究脂多糖(LPS)对肥大细胞高迁移率族蛋白B1(HMGB1)释放的诱导作用及其胞内信号通路-方法:采用小鼠肥大细胞株P815,观察不同剂量LPS诱导后细胞培养上清液中HMGB1含量和细胞HMGB1 mRNA表达水平的变化,及不同剂量丝裂原活化蛋白激酶(MAPK)信号通路抑制剂(SB203580-SB202190-U0126和PD98059)对LPS诱导后HMGB1胞外释放的影响-HMGB1含量采用酶联免疫吸附试验检测-结果:LPS诱导后细胞培养上清液中HMGB1含量明显升高,并与LPS剂量-诱导时间有关;100 μg/L LPS分别诱导8-24-48 h后,HMGB1 mRNA表达水平明显增强(P < 0.01);SB203580和SB202190对LPS诱导HMGB1释放有明显的抑制作用(P < 0.05),但U0126和PD98059不显示抑制作用-结论:LPS诱导肥大细胞释放HMGB1,其释放机制与胞内p38 MAPK信号通路有关-

    Abstract:

    Objective:To study the extracellular release of high mobility group box 1(HMGB1)in mast cells induced by lipopolysaccharide(LPS)and related intracellular signaling pathways. Methods:The changes of HMGB1 concentration in the culture medium and HMGB1 mRNA expression of P815 mast cells were investigated using enzyme-linked immunosorbent assay(ELISA)and RT-PCR method after stimulation with various concentrations of LPS. The effects of MAPKs pathway inhibitors(SB203580,SB202190,U0126 and PD98059)on extracellular release of HMGB1 were observed in LPS-induced mast cells. Results:LPS induced extracellular release of HMGB1 in mast cells in a dose- and time-dependent manner. The level of HMGB1 mRNA expression significantly was increased by stimulation with 100 μg/L LPS for 8,24 or 48 hours(P < 0.01). SB203580 and SB202190 partly inhibited HMGB1 release in LPS-induced mast cells(P < 0.05). But no effects were observed in U0126 and PD98059 treated group. Conclusion:LPS can induce mast cells to release HMGB1,in which the intracellular p38 MAPK signaling pathway is involved.

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杨〓岚,李〓蕾,邵俊良,许〓飞,陈国千.脂多糖诱导肥大细胞释放HMGB1研究[J].南京医科大学学报(自然科学版),2010,(7):919-922

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  • 收稿日期:2010-01-05
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