骨桥蛋白在大鼠慢性移植肾肾病中的表达及意义
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The association between osteopontin and chronic allograft nephropathy in rats
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    目的:探讨慢性移植肾肾病(chronic allograft nephropathy,CAN)大鼠的组织学改变与骨桥蛋白(osteopontin,OPN)表达的关系-方法:以F344大鼠为供体,Lewis大鼠为受体,行原位异体肾移植,建立大鼠CAN模型,作为CAN组;以Lewis大鼠做供-受体,行原位异体肾移植,作为对照组-12周后,观察两组大鼠肾功能-肾组织学改变;免疫组织化学法检测OPN及转化生长因子-β1(TGF-β1)的表达-结果:与对照组比较,CAN组大鼠24 h尿蛋白定量-血肌酐及尿素氮明显升高,差异有统计学意义(P < 0.01);根据Banff 97评分标准,CAN组和对照组肾小管萎缩评分分别为1.70±0.82-0.10±0.30,肾小管间质纤维化评分分别为2.20±0.79-0.30±0.48,差异均有统计学意义(P < 0.01);CAN组移植肾肾小管上皮细胞中TGF-β1蛋白的阳性表达率为100%,而在对照组移植肾组织中未见阳性表达;CAN组移植肾组织中OPN的阳性表达率为90%,而在对照组移植肾组织中未见阳性表达,两者均存在明显差异-结论:OPN在CAN大鼠移植肾组织中高表达,可能作为TGF-β1的下游分子参与TGF-β1介导的慢性移植肾病变的发生发展-

    Abstract:

    Objective:To investigate the expression of osteopontin in transplanted rat kidney and its relationship with chronic allograft nephropathy(CAN). Methods:Fisher(F344)kidneys were orthotopically transplanted into Lewis rat recipients(CAN group). Lewis to Lewis rat kidney transplantation was served as the control(control group). Renal function and urine protein excretion levels of the rats were analyzed. Animals were sacrificed 12 weeks post-transplantation for histological and immunohistochemical detection. Results:Renal function deteriorated progressively in the CAN group,and there was typical chronic allograft tubulointerstitial fibrosis observed in the kidneys. Immunohistochemistry staining showed that the positive rate of TGF-β1 expression in CAN group was 100%,higher than that in control group(0%). The positive rate of OPN expression in CAN group was 90%,while zero in control group. Compared with control group,the intensity of osteopontin increased along with the progression of CAN in CAN group. Conclusion:Our study suggests that the osteopontin plays an important role in the progression of CAN. The expression of osteopontin in epithelial cells could act as a molecular marker of CAN.

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柏金明,徐东亮,俞〓欣,刘〓敏,鲁〓佩,殷长军,张〓炜,徐正铨,顾〓民.骨桥蛋白在大鼠慢性移植肾肾病中的表达及意义[J].南京医科大学学报(自然科学版),2010,(7):932-935

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  • 收稿日期:2010-01-31
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