血红素氧合酶-1对人胃癌SGC7901细胞5-氟尿嘧啶化疗敏感性的影响
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:


Influence of heme oxygenase-1 on the sensitivity of human gastric cancer cell line SGC7901 to 5-FU in vitro
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:体外观察血红素氧合酶-1(heme oxygenase-1,HO-1)对人胃癌细胞SGC7901 5-氟尿嘧啶(5-FU)化疗敏感性的影响及其可能的机制-方法:应用不同浓度锌原卟啉(ZnPP)-氯化血红素(Hemin)单独或联合5-FU作用于人胃癌SGC7901细胞不同时间,四甲基偶氮唑盐(MTT)法观察药物对细胞生长抑制作用;Western blot分析细胞HO-1蛋白表达变化;流式细胞术检测细胞凋亡率;活性氧(ROS)检测试剂盒分析其凋亡机制-结果:ZnPP和5-FU均可明显抑制人胃癌SGC7901细胞生长,呈剂量依赖性;5-FU联用ZnPP(10 μmol/L)与单用药相比,明显提高了细胞生长抑制率和凋亡率(P < 0.05),联用Hemin(10 μmol/L)则明显降低细胞生长抑制率和凋亡率(P < 0.05);人胃癌SGC7901细胞中可见HO-1表达,单用5-FU或Hemin后均能使其表达增强,联用ZnPP可逆转此现象;单用5-FU与对照组比较,细胞内ROS活性明显增高,联用后ZnPP可进一步提高细胞内ROS活性(P < 0.05),而联用Hemin明显降低细胞内ROS活性水平(P < 0.05)-结论:ZnPP抑制HO-1表达,能够增强人胃癌SGC7901细胞对5-FU化疗敏感性,这一作用可能与增加细胞内ROS活性有关-

    Abstract:

    Objective:To study the potential effects of heme oxygenase-1(HO-1)on the sensitivity of human gastric cancer cell line SGC7901 to 5-FU in vitro and the possible mechanisms involved. Methods:All cells were treated with various concentrations of hemin,ZnPP and 5-FU combination or alone at different times. The inhibition rate of cells was evaluated by MTT assay. Western blot was used for the detection of HO-1 expression. Flow cytometry was performed to detect cell apoptosis. ROS activity was examined by ROS colorimetric assay. Results:The application of ZnPP or 5-FU significantly inhibited the proliferation of human gastric cancer cells and the effect was dose-dependent.ZnPP(10 μmol/L)combined with 5-FU led to more inhibition and cell apoptosis than ZnPP or 5-FU alone(P < 0.05).On the other hand,hemin(10 μmol/L)markedly increased the resistance of SGC7901 cells to 5-FU-mediated cytotoxicity(P < 0.05). 5-FU or hemin increased HO-1 protein expression compared with control group,and the increase was reversed by the use of ZnPP. The activity of ROS in 5-FU plus ZnPP group had a significant increase compared with other groups(P < 0.05),and the increase was reversed by the addition of hemin. Conclusion:ZnPP can enhance the chemosensitivity of SGC7901 cells to 5-FU,in which the inhibition of HO-1 expression and elevation of intracellular ROS activity may be involved.

    参考文献
    相似文献
    引证文献
引用本文

武正山,张〓峰.血红素氧合酶-1对人胃癌SGC7901细胞5-氟尿嘧啶化疗敏感性的影响[J].南京医科大学学报(自然科学版),2010,(7):946-950

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2010-01-11
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明