Objective:To compare regional variations of retina macular thickness after near reading performance under binocular viewing conditions in emmetropic and myopic subjects. Methods:Retina macular thicknesses of 9 different areas in the right eye were measured in emmetropes and myopes before and after binocular near reading using the by optic coherence tomography(OCT). Results:There was no significant difference in central and inner ring(1~3 mm)macular thicknesses and in volumes between emmetropic and myopic eyes(P > 0.05). The myopic subjects had significantly lower macular thickness than that of the emmetropic subjects in temporal outer(213.0±12.6 vs 225.1±9.5 μm P < 0.05),nasal outer(260.3±14.5 vs 272.3±7.2 μm P < 0.05),and inferior outer quadrant(224.6±12.9 vs 238.9±8.2 μm P < 0.05). The macular thickness had significantly changes after binocular reading task in myopic temporal outer quadrant,while had no changes in emmetropic eye. Conclusion:Retinal thicknesses are related to refractive status in normal subjects with regional variations in correlation within macular region. Regional variations of retina macular changes were found binocular near reading performance in emmetropia and myopia.