黄芪调节哮喘失衡表达转录因子T-bet/GATA-3的研究
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无锡市科技局项目(CSZ200716)


The effects of Astragalus membranaceus on imbalanced T cell-specific transcription factors T-bet and GATA-3 in asthma
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    摘要:

    目的:观察T细胞特异转录因子T-bet/GATA-3在支气管哮喘中失衡表达,探讨黄芪对其调节作用-方法:50只雄性SPF级SD大鼠随机分为对照组(C组)-哮喘组(A组)-黄芪低(H1组)-中(H2组)-高(H3组)剂量组,20只雄性SPF级致敏SD大鼠脾脏中分离获得CD4+T细胞,分为对照组(C组),OVA干预组(A组),黄芪干预组低(H1组)-中(H2组)-高(H3组)-HE染色观察气道病理改变;酶联免疫吸附测定(ELISA)法检测细胞因子(IL)-4,5和IFN-γ含量;逆转录-聚合酶链反应(RT-PCR)测定法检测T-bet和GATA-3 mRNA表达;免疫印迹(Western blot)法检测T-bet和GATA-3蛋白表达-结果:在大鼠肺组织中嗜酸粒细胞(EOS)-淋巴细胞-管壁面积/支气管管腔内周长(WA/Pi)和支气管平滑肌面积/支气管管腔内周长(ASM/Pi)A组比C组明显增多或增厚(P均< 0.01),H1-H2-H3组中不同程度的减少或减轻(P > 0.05);血清中IL-4和IL-5含量A组高于C组(P均< 0.01),H1-H2-H3组剂量组均低于A组(P均< 0.01),血清中IFN-γ含量A组远低于C组(P均< 0.01),H1-H2-H3组明显高于A组(P均< 0.01),CD4+T细胞培养上清液中H1-H2-H3组IL-4和IL-5含量均降低,同时增加IFN-γ含量,与A组比较,差异均有统计学意义(P均< 0.01);肺组织和CD4+T细胞中T-bet mRNA和蛋白表达H1-H2-H3组高于A组(P均< 0.01);而GATA-3mRNA和蛋白表达H1-H2-H3组低于A组(P均< 0.01)-结论:T细胞特异转录因子T-bet/GATA-3在哮喘中失衡表达,黄芪抑制哮喘气道炎症可能通过双向调节T-bet和GATA-3平衡来实现

    Abstract:

    Objective:To observe the imbalanced T cell-specific transcription factors T-bet and GATA-3 in asthmatic rats and discuss the regulation of the Astragalus membranaceus acts on them. Methods:Fifty male SD rats were randomly divided into Group C(control group),Group A(asthmatic group),Group H1(low-dose Astragalus membranaceus group),Group H2(medium-dose Astragalus membranaceus group) and Group H3(high-dose Astragalus membranaceus group),which were observed the changes of airway histology. CD4+T cells were isolated from twenty male SD rats spleens and incubated with RPMI-1640 at 37℃ for 24 hours,and were divided into 5 groups as noted above. The concentrations of interleukin(IL)-4,IL-5,IFN-γ in serum and CD4+T cells supernatant were measured by enzyme-linked immunosorbent assay(ELISA),while the mRNA expressions of T-bet and GATA-3 were detected by reverse transcription-polymerase chain reaction(RT-PCR),and the protein expressions of T-bet and GATA-3 were detected by western blot respectively. Results:The numbers of eosinophils(EOS) and lymphocytes,the thicknesses of WA/Pi and ASM/Pi of Group A,were significantly elevated compared with those of Group C(P < 0.01). The EOS and lymphocytes,the thicknesses of WA/Pi and ASM/Pi of Group H1-3 were lower than those of Group A. The IL-4 and IL-5 concentrations of Group A were significantly elevated compared with those of Group C,and those of Group H1-H3 were lower than Group A(P < 0.01). Compared with Group C(P < 0.01),the significantly lower concentration of IFN-γ in Group A was detected,and the Group H1-3 had the higher concentrantions of IFN-γ than Group A(P < 0.01). In CD4+T cells supernatant,the concentrations of IL-4 and IL-5 were distinctly decreased and the concentrantions of IFN-γ were increased in the Group H1-3. There were obvious differences in the concentrations of IL-4,IL-5 and IFN-γ between the Group H1-3 and Group A(P < 0.01). In Group H1-3,the expressions of T-bet mRNA and protein in lungs and CD4+T cells were obviously increased compared with those of Group A (P < 0.01);and the Group H1-3 has the lower levels of GATA-3 mRNA and protein expressions than Group A(P < 0.01). Conclusion:In asthma the imbalanced T cell-specific transcription factors T-bet and GATA-3 play a key role in Th2 polarization with over-production of Th2-like cytokines. Astragalus membranaceus inhibits the asthmatic airway inflammation with regulating the balance of transcription factors T-bet and GATA-3.

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赵〓薇,卞〓涛,吴〓艳,陆国础,惠复新,高〓霏.黄芪调节哮喘失衡表达转录因子T-bet/GATA-3的研究[J].南京医科大学学报(自然科学版),2010,(9):1249-1254

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  • 收稿日期:2010-03-20
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