Objective:To study the feasibility of selective extraction of chemical constituents from Polygonum cuspidatum with β-cyclodextrin. Methods:Chemical constituents of Polygonum cuspidatum were selectively extracted by crystallization of β-cyclodextrin inclusion complex in aqueous solution. Results:Six constituents in complex were shown by HPLC spectroscopy. Six compounds were isolated by semi-preparative reversed phase liquid chromatography and recrystallized. Five of the six compounds were identified:emodin, physcion, resveratrol, emodin-8-O-β-D-glucopyranoside and polydation. Conclusion:Chemical constituents of Polygonum cuspidatum could be selectively extracted with β-cyclodextrin. This method is a new way of extraction of chemical constituents from Chinese Herbal Medical.