Objective: To investigate the effects of dendritic cells(DCs) loaded with pancreatic cancer cells antigen on the immune function of the patients with unresectable pancreatic cancer. Methods:DCs isolated from peripheral blood mononuclear cell of pancreatic cancer patients were cultured and stimulated using rhGM-CSF and rhIL-4 in vitro,and then were pulsed with pancreatic cancer cells antigen. Thirty patients with unresectable pancreatic cancer were randomly divided into 2 groups. The dendritic cell vaccine was used in 15 cases(group A),and chemotherapy was applied in 15 cases(group B) as controls. Immune function and clinical effects were compared between the two groups. Results:The levels of CD3+-CD3+CD4+-CD4+/CD8+ and NK cell in group A were increased after vaccination(P < 0.05). So did the levels of IL-2,IL-12,IFN-γ(P < 0.05). Conclusion:The DCs vaccines loaded with pancreatic cancer cells antigen could improve the immune function of the patients with unresectable pancreatic cancer.