Objective: To detect the presence of common viral pathogens in clinical specimens of respiratory tractand to understand the current situation of viral infection, children’s acute respiratory tract infection(ARTI) in Nanjing Children＇s Hospital. Methods: Deep nasopharyngeal secretions(NPS) specimens were collected in Hospitalized children diagnosed with ARTI. A variety of viral pathogens including respiratory syncytial virus(RSV), influenza virus(IFV), parainfluenza virus(PIV), human metapneumovirus (HMPV), adenovirus (ADV), rhinovirus (HRV) and enterovirus(EV) were detected using reverse transcription polymerase chain reaction(RT-PCR). Meanwhile, the clinical and epidemiological manifestations of ARTI caused by these viral pathogens were analyzed. Results:A total of 212 cases affected with viral pathogens were detected in 340 specimens (62.35%). Among them, 132 cases were RSV infection (62.3%), 45 cases IFV infection (21.3%), 13 cases PIV infection (6.1%), 13 cases HMPV infection (6.1%), 4 cases ADV infection (1.9%), 2 cases HRV Infection (0.9%), and 7 cases EV infection (3.3%). Conclusion:RSV infection is most common in ARTI children. Few children have multiple viral infections.