Objective: To evaluate the correlation between the utilization of first-generation cephalosporins antibiotics and resistance rates of staphylococcus aureus(MSSA) from 2001 to 2009, to provide theoretical evidence for clinical antimicrobial medication choice. Methods:DDDs(DDDs/1000) of first-generation cephalosporins antibiotics and resistance rates of MSSA were counted, and correlation of two variables was analyzed by Sperman’s non-parametric correlation using SPSS17.0 software. Results:During 9 years there was overall upward trend in resistance rates of MSSA. The resistance rates of penicillin, cefuroxime were the highest, while resistance rate of glycopeptide was lowest. Resistance of co-trimoxazole, minocycline had decreased, including DDDs of first-generation cephalosporins was decreased year by year. There were respectively perfectly positively correlation between DDDs of first-generation, cefradine and cefadroxil and resistance rate of minocycline(r=1.000,P < 0.01).DDDs of cephradine and first-generation cephalosporins and resistance rates of penicillin, cefepime, clindamycin and amikacin were respectively negatively correlated(r= -0.900,P < 0.01;r=-0.967,P < 0.01;r = -0.967, P < 0.01;r=-0.917,P < 0.01;r=-0.817,P < 0.01;r=-0.733,P < 0.05;r=-0.950,P < 0.01;r=-0.783,P < 0.05);The DDDs of cephradine and resistance rates of piperacillin /tazobactam, cefazolin, erythromycin, levofloxacin were negatively correlated(r=-0.683,P < 0.05;r=-0.917,P < 0.01; r=-0.750,P < 0.05;r=-0.881,P < 0.01 respectively).The DDDs of cefadroxil and resistance rates of cefuroxime were perfectly positively correlated(r=1.000,p < 0.01). Conclusion: The DDDs of first-generation cephalosporin and resistance rate of MSSA were significantly correlat, and clinical doctors should pay more attention on the usage of cephalosporin antibiotics.