骨髓细胞不同组份形成成纤维细胞集落形成单位的效率及1,25(OH)2D3和PGE2在其中的调节作用
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教育部高等学校博士学科点专项科研基金(20070312010)


Efficiency of colony forming unit-fibroblastic formed by the different fraction of bone marrow cells and regulation of 1,25(OH)2D3 and PGE2 in colony formation
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    摘要:

    目的:研究骨髓间充质干细胞(BM-MSCs)是否存在于骨髓不同组份中,以及其成纤维细胞集落形成单位(CFU-f)形成效率是否受1,25二羟基维生素D3[1,25(OH)2D3]和前列腺素E2(PGE2)的调节-方法:将总骨髓细胞和培养1天后的非黏附骨髓细胞经梯度密度离心分为单核细胞和粒细胞/红细胞两组份或单核细胞-粒细胞和红细胞三组份,在缺乏或添加10-8 mol/L 1,25(OH)2D3或10-7 mol/L PGE2条件下贴壁培养10天,行亚甲基蓝染色,计数CFU-f 数目-结果:①单核细胞组份CFU-f形成效率约占总CFU-f的10%,粒细胞/红细胞组份-粒细胞组份-红细胞组份CFU-f形成效率分别约占总CFU-f的90%-47%-35%,均明显高于单核细胞组份;②非黏附骨髓细胞来源的不同组份CFU-f形成效率占总CFU-f的71%,较总骨髓来源不同组份CFU-f形成效率明显增加;③1,25(OH)2D3和PGE2能够明显刺激总骨髓来源的单核细胞和粒细胞组份的CFU-f形成效率以及非黏附骨髓细胞来源的单核细胞-粒细胞和红细胞组份的CFU-f形成效率-结论:骨髓细胞各组份中都存在BM-MSCs,1,25(OH)2D3和PGE2都具有促进BM-MSCs扩增的作用-

    Abstract:

    Objective: To determine whether bone marrow mesenchymal stem cells (BM-MSCs) are presented in the different fractions of bone marrow cells and whether the efficiency of colony forming unit-fibroblastic(CFU-f) formed by BM-MSCs is regulated by 1,25(OH)2D3 and prostaglandin E2(PGE2). Methods:Total bone marrow cells or non-adherent bone marrow cells derived from total bone marrow cell cultures for 1 day were separated into the mononuclear cell fraction and the granulocyte/erythrocyte fraction or the mononuclear cell fraction, the granulocyte fraction and the erythrocyte fraction by density-gradient centrifugation and were cultured in the absence or presence of 10-8mol/L 1,25(OH)2D3 or 10-7 mol/L PGE2 for 10 days. The resulting cells were stained with methylene blue and the number of CFU-f was counted. Results:①The CFU-f formed by mononuclear cell fraction accounted for about 10% of the total CFU-f, and CFU-f formed by granulocyte/erythrocyte fraction, granulocyte fraction and erythrocyte fraction accounted for about 90%,47% and 35% of the total CFU-f, respectively; ②The fractions derived from the non-adherent bone marrow cells accounted for about 71% of the total CFU-f, which was more than those formed by the fractions derived from total bone marrow cells; ③ Treatment of 1,25 (OH)2D3 and PGE2 enhanced the CFU-f formation of the mononuclear fraction and the granulocyte fraction derived from total bone marrow cells and the mononuclear cell fraction, the granulocyte fraction and the erythrocyte fraction derived from non-adherent bone marrow cells. Conclusion: Our results indicate that BM-MSCs are presented in the different fractions of bone marrow cells,and 1,25(OH)2D3 and PGE2 play a role in stimulating proliferation of BM-MSCs.

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王 嵘,苗登顺,季 吉.骨髓细胞不同组份形成成纤维细胞集落形成单位的效率及1,25(OH)2D3和PGE2在其中的调节作用[J].南京医科大学学报(自然科学版),2011,(1):8-12

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  • 收稿日期:2010-07-19
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