下丘脑室旁核组胺H3受体对哮喘大鼠下丘脑-垂体-肾上腺轴功能的调控
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Effect of histamine H3 receptor in the hypothalamic paraventricular nuclei on hypothalamic-pituitary-adrenal axis in rats with asthma
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    摘要:

    目的: 探讨哮喘大鼠下丘脑室旁核(paraventricular nuclei,PVN)内组胺H3受体对下丘脑-垂体-肾上腺轴(HPA)功能的调控-方法:根据Elwood方法,制备哮喘模型-采用中枢立体定位技术,在PVN内微量注射组胺H3受体激动剂R-(α)-甲基组胺[R-(α)-methylhistamine,R-(α)-MeHA] 2 μg或组胺H3受体拮抗剂Thioperamide 5 μg, 注射药物体积均为1 μl-高效液相色谱法检测PVN内组胺含量;放射免疫分析方法检测正中隆起中促肾上腺皮质激素释放激素(CRH)和外周血中促肾上腺皮质激素(ACTH)-皮质酮(CORT)含量;酶联免疫吸附法(ELISA)检测脑组织和血浆IL-10含量-结果:与生理盐水组相比,R-(α)-MeHA 组大鼠PVN内组胺含量明显下降(P < 0.01); CORT-ACTH及 CRH含量明显增加 (P < 0.01); IL-10含量增高(P < 0.01);Thioperamide可阻断上述效应,差异均有统计学意义-结论:大鼠PVN内组胺H3受体参与调控哮喘的发作,该作用可能与其影响HPA轴功能有关-

    Abstract:

    Objective: To determine whether histamine H3 receptor in the hypothalamic paraventricular nuclei (PVN) regulates the function of hypothalamic-pituitary-adrenal (HPA) axis in rats with asthma. Method: Rat model for asthma was established by Elwood’s methods. A stainless steel jacket was implanted into the PVN using a technique of central three-dimensional position, and R-(α)-methylhistamine [R-(α)-MeHA, a H3 receptor agonist] 2 μg or thioperamide (a H3 receptor antagonist) 5 μg was injected into the PVN. Levels of histamine in PVN were detected by high-performance liquid chromatography, and the concentrations of CRH in median eminence, ACTH and CORT in plasma were detected by the radioimmunoassay. Relative levels of IL-10 in both hypothalamus tissue and plasma were measured by double-ligand-linked immunosorbent assay. Result: Levels of histamine in PVN decreased significantly, whereas the concentrations of CRH in median eminence, ACTH and CORT in plasma and the levels of IL-10 in both hypothalamus tissue and plasma were all increased significantly in acute episode asthma rats with treatment of R-(α)-MeHA compared with control rats, and these responses were blocked by treatment of thioperamide, a H3 receptors specific antagonist. Conclusion: Specific activation histamine H3 receptors in PVN can improve the function of HPA in rats with asthma.

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季 吉,董 榕.下丘脑室旁核组胺H3受体对哮喘大鼠下丘脑-垂体-肾上腺轴功能的调控[J].南京医科大学学报(自然科学版),2011,(1):40-43

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  • 收稿日期:2010-07-01
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