Objective:To investigate the mechanism of M-譈ller cell adhesion to vitreous gel. Methods:Porcine M-譈ller cells were extracted and cultured,identified with immuno-recognition with glial fibrillary acidic protein(GFAP),vimentin and glutamine synthetase(GS) antibody. Cells were collected and incubated with/without integrinα2β1 and CD44 antibody before application to fresh porcine vitreous for 2 hours,and then washed with PBS for 3 times. Remaining cells on the vitreous were counted. Results:① Cells collected were 97.7% positive for GFAP,94.6% positive for vimentin,86.0% positive for GS,respectively. Cells adhered to vitreous after 2 hours of culture,formed net-like structure and enter vitreous gel after 72 hours;② Pre-incubation with integrinα2β1 antibody significantly decreased the remaining cell,while no such significance was observed in the cells incubated with CD44 antibody. Conclusion:M-譈ller cell can adhere to vitreous and proliferate,in which the interaction with type Ⅱ collagen is an important mechanism.