2型糖尿病患者皮下?大网膜脂肪组织脂联素mRNA表达变化及意义
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南京医科大学重点课题基金资助(07NMUZ014)


Expression and significance of adiponectin mRNA in subcutaneous and omental adipose tissue of type 2 diabetic patients
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    目的:观察2型糖尿病患者皮下及大网膜脂肪组织脂联素(adiponectin)表达水平的变化,及其与血清脂联素-体质指数(BMI)-腰围(WC),腰/臀比值(WHR)-收缩压(SBP)-舒张压(DBP)-空腹血糖(FPG)-糖化血红蛋白(HbA1c)-总胆固醇(TC)-甘油三酯(TG)-低密度脂蛋白胆固醇(LDL-C)-高密度脂蛋白胆固醇(HDL-C)-尿酸(UA)等相关指标的关系,探讨脂联素在2型糖尿病患者发生胰岛素抵抗中的作用-方法:用半定量RT-PCR方法检测23例2型糖尿病合并肥胖患者(DM+FAT)-23例2型糖尿病患者(DM)-33例非糖尿病肥胖患者(control+FAT)-23例非糖尿病患者(control)腹部皮下及大网膜脂肪组织脂联素mRNA的表达水平,并测量身高-体重-腰围-臀围-血压-FPG-HbA1C-血脂-尿酸-血清脂联素,并计算BMI-WHR-结果:①糖尿病肥胖组SBP-DBP-FPG-HbA1c-TG-LDL-C-尿酸均高于对照肥胖组,HDL-C-皮下及大网膜脂肪组织脂联素mRNA表达量-血清脂联素均低于对照肥胖组(P < 0.01)-两组的大网膜脂联素表达量低于其皮下表达量(P < 0.01);②糖尿病非肥胖组的FPG-HbA1c-TG-尿酸均高于对照非肥胖组,HDL-C-皮下及大网膜脂肪组织脂联素mRNA表达量-血清脂联素均低于对照非肥胖组(P < 0.01)-两组的大网膜脂联素表达量低于其皮下表达量(P < 0.01);③糖尿病肥胖组的BMI-WHR-SBP-DBP-FPG-HbA1c-TG-尿酸均高于糖尿病非肥胖组,大网膜脂肪组织脂联素mRNA表达量-血清脂联素均低于糖尿病非肥胖组(P < 0.01);④直线相关分析显示,糖尿病肥胖组的网膜脂联素mRNA的表达水平与BMI-FPG(P < 0.01)-WHR-WC(P < 0.05)呈负相关,与血清脂联素水平呈正相关(P < 0.01)-结论:2型糖尿病合并肥胖患者大网膜脂肪组织脂联素mRNA表达显著降低,血清脂联素水平显著降低;BMI-WC-WHR-FPG等临床指标可间接反映胰岛素抵抗程度-

    Abstract:

    Objective: To observe the adiponectin mRNA expression in subcutaneous and omental adipose tissue of type 2 diabetic patients and their relation with serum adiponectin, body mass index(BMI), waist circumference(WC),waist hip ratio(WHR), blood pressure, fasting plasma glucose(FPG), HbA1c, blood lipids and uric acid(UA). Methods:Twenty-three T2DM patients with obesity, 23 T2DM patients, 33 obesity patients and 23 controls were selected. The adiponectin mRNA expression level in adipose tissue was assayed by RT-PCR. The level of serum adiponectin was measured with ELISA kit. FBG, triglyceride(TG), total cholesterol(TC), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), HbA1c, UA of all subjects were determined and their blood pressure, height, weight, waist circumference, hip circumference were measured to calculate BMI,WHR. Results:①The SBP, DBP, FPG, HbA1c, TG, LDL-C, UA were significantly higher, and serum adiponectin, HDL-C and adiponectin mRNA expression level in both subcutaneous and omental adipose tissue were significantly lower in diabetic obesity group than in obesity group(P < 0.05), the adiponectin mRNA expression level in omental were significantly lower than in subcutaneous adipose tissue in both groups(P < 0.01). ②The FPG, HbA1c, TG, UA were higher, serum adiponectin, HDL-C and adiponectin mRNA levels expressed in both subcutaneous and omental adipose tissue were lower in diabetic group without obesity than in control group without obesity(P < 0.05), the adiponectin mRNA expression level in omental was lower than in subcutaneous adipose tissue in both groups(P < 0.01). ③The BMI,WHR,SBP,DBP,FPG,HbA1C,TG,UA were higher, serum adiponectin and adiponectin mRNA level expressed in both subcutaneous and omental adipose tissue were lower in diabetic obese group than in diabetic group without obesity(P < 0.05). ④The adiponectin mRNA expression level in omental adipose tissue of diabetic obesity group was negatively correlated to BMI,WHR,FPG(P < 0.05), and was positively correlated to serum adiponectin(P < 0.01). Conclusion:T2DM patients with obesity show down-regulated expression of adiponectin mRNA in omental adipose tissue and lower serum adiponectin than control obese group,BMI,WC,WHR,FPG are correlated with adiponectin mRNA expression and insulin resistance.

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李倩倩,蒋秀琴,李慧敏,缪 珩.2型糖尿病患者皮下?大网膜脂肪组织脂联素mRNA表达变化及意义[J].南京医科大学学报(自然科学版),2011,(3):404-409

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  • 收稿日期:2010-09-17
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