TLR7/8配体R-848体外抑制小鼠纯化B细胞产生IgE的机制研究
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广州市属高校科研项目基金资助(10A159)


The mechanism of inhibition of IgE production of TLR7/8 ligand R-848 by murine purified B cells stimulated with anti-CD40 plus IL-4 in vitro
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    摘要:

    目的:观察TLR7/8配体R-848对小鼠纯化B细胞产生IgE的调节作用及其可能机制-方法:制备小鼠脾细胞悬液,用磁珠分选出脾细胞中的CD19+B细胞;纯化的B细胞在基础培养液-含anti-CD40+IL-4加或不加R-848的培养液:采用ELISA的方法检测IgE的浓度;流式细胞术检测B细胞的活化情况;CFSE标记B细胞,培养后检测细胞的分裂情况-结果:R-848以剂量依赖方式抑制anti-CD40+IL-4诱导的B细胞产生IgE,增强anti-CD40+IL-4诱导的B细胞的活化-R-848能够直接作用于B细胞,抑制anti-CD40+IL-4诱导的B细胞分裂-结论:R-848抑制B细胞产生IgE,可能与其抑制anti-CD40+IL-4诱导的B细胞的分裂有关-

    Abstract:

    Objective:To investigate the regulatory role of TLR7/8 ligand R-848 on IgE production of murine purified B cells. Methods:CD19+B cells were purified with MACS from splenocytes of murine, and cultured with base medium, and medium with anti-CD40 plus IL-4 in the presence or absence of R-848. The concentration of IgE was determined with ELISA,and the activation of B cells was determined with FACS. Purified B cells were labeled with CFSE, and after culturing the cells were harvested and the division of cells was determined with FACS. Results:R-848 could inhibit IgE production of purified B cells stimulated with anti-CD40 plus IL-4 in a dose dependent manner, and enhance the activation of B cells. Moreover, R-848 could inhibit the division of B cells stimulated with anti-CD40 plus IL-4. Conclusion:The inhibitory role of R-848 on IgE production might be related with a reduction in B cell division.

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沈二霞,吴长有. TLR7/8配体R-848体外抑制小鼠纯化B细胞产生IgE的机制研究[J].南京医科大学学报(自然科学版),2011,(5):640-644

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  • 收稿日期:2010-03-21
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