抗人滋养层细胞表面抗原-2单抗的制备及免疫学特性分析
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南京市医学科技发展重点项目(ZKX09015);南京市科技发展项目(200901083)


Preparation and immunologic characterization of a monoclonal antibody against human trophoblast cell-surface antigen 2
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    摘要:

    目的:利用杂交瘤技术制备抗人滋养层细胞表面抗原-2(trophoblast cell-surface antigen 2,Trop2)单克隆抗体并鉴定其免疫学特性-方法:以胰腺癌细胞系BxPC3免疫BALB/c小鼠,取其脾细胞与SP2/0细胞融合后制备单抗-通过酶联免疫法-免疫荧光-免疫沉淀和质谱分析-流式细胞术-免疫组织化学等方法,鉴定单抗的免疫学特性-结果:通过细胞的融合与筛选,获得了持续分泌抗Trop2单抗的杂交瘤细胞株,该抗体可以识别细胞表面的Trop2膜蛋白,也可用于免疫组化识别人肿瘤组织中的Trop2蛋白,同时该抗体对乳腺癌细胞的生长具有一定的抑制作用-结论:该单抗具有作为Trop2阳性表达肿瘤靶向治疗的潜在应用价值-

    Abstract:

    Objective:To produce a monoclonal antibody (mAb) against human trophoblast cell-surface antigen 2(Trop2) with hybridoma technology and identify its immune specificity. Methods:The spleen cells from BALB/c mice immunized with pancreatic cancer cell line BxPC3 cells were fused with SP2/0 cells to produce a monoclonal antibody against human Trop2. Further, several immunological methods such as ELISA,IF,IP,MALDI-TOF MS,FACS and IHC were used to characterize the immune specificity of the mAb. Results:The results demonstrated that the specific mouse anti-human Trop2 mAb was successfully produced and can be secreted continuously and steadily. Moreover, this mAb can effectively recognize the transmembrane glycoprotein Trop2 over-expressed in human cancer cells and tissues. Conclusion:We successfully obtained mouse anti-human Trop2 mAb which may be potentially utilized for some tumor targeted therapy.

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梁 洁,刘琼琼,张慧林,林 红,唐 奇,刘 玉,苏亦平,冯振卿,朱 进.抗人滋养层细胞表面抗原-2单抗的制备及免疫学特性分析[J].南京医科大学学报(自然科学版),2011,(5):645-650

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  • 收稿日期:2010-12-07
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