C反应蛋白对小鼠主动脉内皮细胞胞内游离钙的影响
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国家自然科学基金资助项目(30770894)


Effect of CRP on intracellular free calcium in mice aorta endothelial cells
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    摘要:

    目的:研究C反应蛋白(C-reactive protein,CRP)对小鼠主动脉内皮细胞胞内游离钙[Ca2+]i的作用-方法:原代培养小鼠主动脉内皮细胞,取生长状态良好的内皮细胞分为对照组(未加CRP)和不同浓度CRP处理组,25 min后观察各组内皮细胞[Ca2+]i-将内皮细胞用1 μmol/L阿托伐他汀预处理30 min后,加入100 mg/L CRP,观察阿托伐他汀对CRP作用的影响-结果:与对照组相比,CRP可显著升高内皮细胞胞内[Ca2+]i并呈剂量依赖关系;阿托伐他汀可明显降低CRP引起的[Ca2+]i升高-结论:CRP可导致和加重小鼠主动脉内皮细胞的炎症反应,阿托伐他汀可部分拮抗CRP的致炎作用-

    Abstract:

    Objective:To investigate the effect of CRP on the [Ca2+]i in mice aorta endothelial cells(ECs). Methods:Mice aortic endothelial cells were isolated and cultured. ECs were divided into control group and CRP treated group,and the changes of [Ca2+]i was examined after 25 min. For the effect of atorvastatin,the cells were pre-incubated 30 min by atorvastatin(1 μmol/L)prior to adding CRP(100 mg/L). Results:[Ca2+]i of ECs significantly increased after the treatment of CRP in a dose dependent manner. Furthermore,atorvastatin partially abrogated the up-regulation of [Ca2+]i. Conclusion:Inflammatory of ECs was up-regulation in response to CRP stimulation. Meanwhile,the research also demonstrated that atorvastatin partial suppressed inflammatory induced by CRP treatment.

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刘 强,张丹丹,徐晋妉,卞智萍,吴恒芳,顾春荣,陈相健,杨 笛. C反应蛋白对小鼠主动脉内皮细胞胞内游离钙的影响[J].南京医科大学学报(自然科学版),2011,(5):679-683

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  • 收稿日期:2010-11-10
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