脂多糖致新生鼠脑白质软化模型的建立
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江苏省卫生厅面上科研项目(H200642)


Neonatal rats model of periventricular leukomalacia caused by lipopolysaccharide
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    目的:证实脂多糖致新生鼠脑白质软化模型的可靠性-方法: 16只健康孕鼠随机分为实验组和对照组,于妊娠第15天按0.3 mg/kg剂量脂多糖(lipopolysaccharide, LPS)对实验组孕鼠进行腹腔内注射, 对照组孕鼠在同一天使用等量蒸馏水腹腔内注射,妊娠第21天,孕鼠均分娩,取两组新生鼠脑组织进行形态学观察和脑组织含水量测定-结果:①光镜下可见,实验组脑室周围白质水肿软化,带状或灶状凝固性坏死,而对照组无明显形态学变化;②电镜下发现, 实验组少突胶质细胞线粒体肿胀明显-线粒体脊减少或空泡化,内质网明显扩张,对照组无明显变化;③实验组脑组织含水量较对照组脑组织含水量明显增高,差异具有统计学意义(P < 0.05)-结论:脂多糖可导致新生鼠脑室周围白质软化, 且脑组织含水量也明显增高-脂多糖是制备新生鼠脑白质软化的一种有效方法-

    Abstract:

    Objective:To comfirm the reliability for the neonatal rats model of periventricular leukomalacia caused by lipopolysaccharide. Methods:Sixteen mature pregnant wistar rats were divided into experimental(n=8)and control groups(n=8).On 15 days of gestation, LPS(0.3 mg/kg)and distilled water were injected intraperitoneally to experimental(n=8)group and control group, respectively. Seventy neonatal rats of experimental group and eighty-two in the control group were obtained till the gestation day of 21. Morphological changes and water content in brain tissues were studied. Results:①Periventricular leukomalacia,band-like and spotty coagulation necrosis were found in brain of neonatal rats in the experimental group, but not in the controls; ②Oligodendrocytes, mitochondrial obvious swelling , ridge decreased or vacuolization and endoplasmic reticulum expansion were observed in brain of neonatal rats, in the experimental group, but not in the controls;③Water content in neonatal rats' brain of the experimental group[(89.92±4.06)%]was significantly higher than that in the control group[(87.78±5.21)%](P < 0.05). Conclusion:Lipopolysaccharide given to maternal rats could result in periventricular leukomalacia and increase the water content in the brain tissues of neonatal rats.

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郭 凯,浦潇丹,王艳萍,杨 洋,郭 梅,周晓玉.脂多糖致新生鼠脑白质软化模型的建立[J].南京医科大学学报(自然科学版),2011,(5):694-696

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  • 收稿日期:2010-11-16
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