紫杉醇对TGF-β1促人Tenon’s 囊成纤维细胞增殖的影响
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江苏省教育厅自然科学基金 (07KJB310070)


Effect of paclitaxel on proliferation of human Tenon’s capsule fibroblasts stimulated by TGF-β1
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    摘要:

    目的:研究转化生长因子-β1(transforming growth factor-β1,TGF-β1)促人Tenon’s囊成纤维细胞(human Tenon’s capsule fibroblasts, HTFs)的增殖作用及紫杉醇对该增殖作用的影响-方法:用TGF-β1(0~50 ng/ml)作用于体外培养的HTFs,CCK-8法检测其增殖;用紫杉醇(0~10 000 nmol/L)作用于HTFs 2~24 h,Hochest 33342染色检测其凋亡-结果:2.5~25.0 ng/ml TGF-β1作用2~24 h均可以促进HTFs增殖,10~10 000 nmol/L紫杉醇能抑制TGF-β1促HTFs的增殖作用,其抑制作用与浓度成正比;Hochest 33342染色证实10~10 000 nmol/L紫杉醇对于增殖的HTFs有促凋亡的作用并与浓度成正比-结论:TGF-β1可刺激HTFs增殖,紫杉醇可抑制TGF-β1促HTFs的增殖作用,可以促进细胞的凋亡-

    Abstract:

    Objective:To investigate the effect of TGF-β1 on the proliferation of human Tenon’s capsule fibroblasts(HTFs) and the effect of Paclitaxel on the proliferation of HTFs stimulated by TGF-β1. Methods: ① HTFs cultured by tissue block adhering wall method were observed by optical microscope and identified by immunocytochemistry methods. ② The 4th and 6th generation of HTFs were incubated with various concentrations of TGF-β1(0,2.5,5,10,25,50 ng/ml)for 24 hours. The proliferation of HTFs was evaluated by CCK-8. ③HTFs were treated with 10 ng/ml TGF-β1 and various concentration of Paclitaxel (0,10,100,1 000,10 000 nmol/L) for 24 h in order observe proliferation. ④ The cell apoptosis was measured by Hochest33342 staining. Results:① The Tenon’s fibroblasts were successfully cultured. The results of vimentin immunocytochemisty staining was positive. ② Treatment of the cells with 2.5~25.0 ng/ml TGF-β1 increased the proliferation of the HTFs. ③ 100~10 000 nmol/L Paclitaxel suppressed the proliferation of the HTFs stimulated by TGF-β1. ④ Hochest staining shows a clear apoptotic features. Paclitaxel treatment resulted in increased number of apoptotic cells in a dose-dependent manner. Conclusion: TGF-β1 may increase the proliferation of HTFs, which could be reduced by Paclitaxel. Paclitaxel could bind to TGF-β1 receptor and interfere in signal transmission and inhibit the scarring after glaucoma filtering surgery.

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王 荔,孙 红,杨 翎,袁志兰.紫杉醇对TGF-β1促人Tenon’s 囊成纤维细胞增殖的影响[J].南京医科大学学报(自然科学版),2011,(6):794-798

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  • 收稿日期:2010-11-13
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