内脂素对人胆管癌细胞QBC939侵袭及增殖影响的体外研究
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Effects of visfatin on the proliferation and invasiveness of human cholangiocarcinoma QBC939 cells in vitro
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    摘要:

    目的:研究人内脂素(visfatin)对胆管癌细胞QBC939体外增殖和侵袭能力的影响,并初步探讨可能存在的机制-方法:培养胆管癌细胞QBC939,在不同浓度的内脂素作用不同时间后,以四甲基偶氮唑盐(MTT)法检测细胞增殖能力,用Transwell小室检测内脂素(200 ng/ml)24 h后对胆管癌QBC939细胞侵袭能力的影响,Western blot检测内脂素对QBC939细胞内基质金属蛋白酶(matrix metalloproteinase,MMP)-2蛋白的表达-结果:内脂素可以促进QBC939细胞的增殖(P < 0.01),且存在剂量依赖性;内脂素(200 ng/ml)处理24 h后,QBC939细胞穿透聚碳酸酯膜的能力与对照组相比显著增强(P < 0.01);Western blot显示内脂素(200 ng/m1)处理24 h后,QBC939细胞MMP-2蛋白的表达明显增高-结论:内脂素可以明显提高人胆管癌细胞QBC939的增殖和侵袭能力,其作用机制可能与MMP-2水平的增高有关-

    Abstract:

    Objective:To investigate the effects of visfatin on the proliferation and invasion of human cholangiocarcinoma QBC939 cells, and explore the possible mechanisms. Methods: QBC939 cells were cultured and treated with visfatin at different concentrations of 25,50,100 and 200 ng/ml for 6,12, and 24 h. QBC939 cells without visfatin treatment were used as control group. The proliferation rate of the QBC939 cells was measured by MTT assay. Transwell chamber assay was performed to determine the effect of visfatin 200 ng/ml on the invasion capability of QBC939 cells for 24 h later, and Western blot was used to observe MMP-2 protein expression. Results: The results showed that visfatin could significantly improve growth and proliferation of QBC939 cells. The effect is in a dose and time-depended by MTT method(P < 0.01). The invasion capability of QBC939 cell was significantly increased after visfatin 200 ng/ml treatment for 24 h(P < 0.01). The expression of MMP-2 was increased in QBC939 cells treated with visfatin. Conclusion: Visfatin significantly increases the proliferation and invasion capabilities of QBC939 cells in vitro. The possible mechanism may be related with up regulation of MMP-2 level.

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张俊华,傅 赞,吴新权,石 毅,白剑峰,孙跃明.内脂素对人胆管癌细胞QBC939侵袭及增殖影响的体外研究[J].南京医科大学学报(自然科学版),2011,(6):809-812

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  • 收稿日期:2010-12-20
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