稳定表达GFP-C-Daam1的MDA-MB-231人乳腺癌细胞系的建立
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金资助(30872926),江苏省博士后科研资助(0902023C),南京医科大学科技发展基金重点项目(2010NJMUZ35)


Establishment of a stable GFP-C-Daam1 MDA-MB-231 breast cancer cell line
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:构建稳定表达GFP和GFP-C-Daam1的MDA-MB-231人乳腺癌细胞系,以便进一步研究Daam1对乳腺癌细胞迁移能力的影响-方法:构建表达GFP和GFP-C-Daam1的慢病毒载体,通过慢病毒感染获取稳定表达目的基因的MDA-MB-231人乳腺癌细胞系-用嘌呤霉素筛选稳定表达的细胞系,Western blot确定重组蛋白表达,并用Boyden chamber小室实验检测细胞运动能力的改变-结果:通过慢病毒感染,建立了稳定表达GFP-C-Daam1的MDA-MB-231人乳腺癌细胞系,该细胞系具备更强的内在运动能力,表明目的基因的表达产物功能正常-结论:用慢病毒载体可以构建稳定表达GFP-C-Daam1的MDA-MB-231人乳腺癌细胞系,从而得到研究活化的Daam1的可靠细胞膜型-

    Abstract:

    Objective:To establish a MDA-MB-231 human breast cancer cell line stably express GFP-C-Daam1, and to investigate the influence of Daam1 on the mobility of breast cancer cells. Methods:The lentivrial vectors containing GFP or GFP-C-Daam1 were constructed and infected human breast cancer cells MDA-MB-231. MDA-MB-231 cells were screened by puromycin and stable expression of GFP or GFP-C-Daam1 was confirmed by Western blotting. Boyden chamber assay was used to test the intrinsic motility of the cells. Results:The cell lines stably expressing GFP or GFP-C-Daam1 were established successfully by the lentivrial infection. The GFP-C-Daam1 expressing cells showed greater intrinsic motility, suggesting the products of the interesting gene were active and functional. Conclusion:MDA-MB-231 breast cancer cell lines stably expressing GFP or GFP-C-Daam1 were successfully established using lentiviral methods. These cell lines can be used as model cells for further study of active Daam1 targeting.

    参考文献
    相似文献
    引证文献
引用本文

李卫星,杜 军,胡圳圳,田寅辉,刘皎婧,朱一超.稳定表达GFP-C-Daam1的MDA-MB-231人乳腺癌细胞系的建立[J].南京医科大学学报(自然科学版),2011,(7):925-929

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2011-02-19
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明