代森锰锌对小鼠生精细胞T型Ca2+通道的作用
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金项目(30771831,81072329)


Effects of mancozeb on T-type calcium channels in mouse spermatogenic cells
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:研究农药代森锰锌(mancozeb)对小鼠生精细胞T型钙电流(ICaT)的影响-方法:采用膜片钳全细胞记录技术观察代森锰锌对急性机械分离的小鼠生精细胞ICaT 的影响-结果:Mancozeb(10-20-40-80-120 μmol/L)呈浓度-电压依赖性抑制小鼠生精细胞钙电流,抑制率分别为(9.93±0.90)%-(20.93±2.50)%-(25.92±2.80)%-(42.64±3.10)%-(56.80±3.20)% (n=6,P<0.05)-Mancozeb 对T型钙通道的半数最大抑制浓度(K50)为35.60 μmol/L-120 μmol/L mancozeb显著改变T型Ca2+通道的激活和失活特性:半数激活电压(V1/2a)和激活斜率因子(κa)分别从(-47.09±1.05)mV和(8.67±0.78) mV变为(-51.46±0.84)mV和(10.56±0.619)mV (n=5, P < 0.05);而半数失活电压(V1/2i)和失活斜率因子(κi)分别从(-62.96±3.36)mV和(9.93±1.41)mV变为 (-64.11±5.55)mV和(13.64±1.95)mV (n=5,P < 0.05)-结论:Mancozeb对生精细胞T型钙通道有抑制作用,且呈浓度依赖性-

    Abstract:

    Objective:To investigate the effect of mancozeb on T-type calcium currents(ICaT) in spermatogenic cells. Methods: Ca2+ currents were obtained in acutely dissociated mouse spermatogenic cells by the whole-cell patch clamp technique and the effects of mancozeb on ICaT were observed. Results:Mancozeb at the concentrations of 10, 20, 40, 80 and 120 μmol/L significantly inhibited ICaT in the mouse spermatogenic cells, with the K50 value of 35.60 μmol/L and the inhibition rates of mancozeb were(9.93±0.90)%,(20.93±2.50)%,(25.92±2.80)%,(42.64±3.10)% and (56.80±3.20)%, respectively(n=6, P < 0.05). Mancozeb of 120 μmol/L significantly changed the activation and inactivation of ICaT: the half activation potential(V1/2a) and the activation steepness factor (κa) from (-47.09±1.05)mV and (8.67±0.78)mV to (-51.46±0.84)mV and (10.56±0.619)mV, respectively(n=5, P < 0.05); the half inactivation potential (V1/2i) and the inactivation steepness factor (κi) from (-62.96±3.36)mV and (9.93±1.41)mV to(-64.11±5.55)mV and (13.64±1.95)mV, respectively (n=5, P < 0.05). Conclusion:Mancozeb has significant inhibitory effects on ICaT in mouse spermatogenic cells with concentration dependence.

    参考文献
    相似文献
    引证文献
引用本文

朱 勤,王 强,王文娟,程 洁,高 蓉,肖 杭.代森锰锌对小鼠生精细胞T型Ca2+通道的作用[J].南京医科大学学报(自然科学版),2011,(7):996-1001

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2011-01-06
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明