肾透明细胞癌中β-环连蛋白抑制基因1启动子CpG岛甲基化与其失表达相关
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Dact1 promoter CpG island methylation was correlated with down-regulation of Dact1 mRNA expression in renal clear carcinoma
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    摘要:

    目的:探讨肾透明细胞癌中β-环连蛋白抑制基因1(dapper homolog 1,antagonist of beta-catenin,Dact1)表达与其启动子CpG岛甲基化状态的关系,并探究他们之间的关系及在肾透明细胞癌发生中的作用。方法:采用逆转录聚合酶链反应(RT-PCR)及甲基化特异性PCR(methylation specific PCR,MSP)分别检测30例癌组织及相应的癌旁对照组织中Dact1基因mRNA的表达和其启动子甲基化状态。结果:癌组织组中,19例Dact1基因mRNA失表达(63%),7例表达低于癌旁对照组(23%);癌旁对照组织组中,2例Dact1基因mRNA低表达(6%)。Dactl基因在癌组织、癌旁对照组织中的甲基化阳性率分别为70%、6%,2组比较差异有统计学意义(P < O.001),Dactl基因mRNA的失表达或低表达与其启动子甲基化状态的相关性分析有统计学意义(P < 0.05)。结论:Dact1基因mRNA的失表达或低表达可能参与了肾透明细胞癌的发生发展,其失表达可能与Dactl启动子区异常甲基化相关。

    Abstract:

    Objective: To discuss the relation between the methylation of Dactl promoter and expression of Dactl mRNA in renal cancer. Methods: The tissue specimens from 30 cases of renal cancer and corresponding nomal tissues were included,and CpG island methylation of Dact1 gene was detected by methylation specific PCR(MSP),and the mRNA expression of Dactl was evaluated by reverse transcription-polymerse chain reaction(RT-PCR). Results: The expression of Dactl mRNA was silenced in 19(63%) and down-regulated in 7(23%) specimens of renal cancer tissues,while down-regulated in 2 (6%) specimens of adjacent control tissues. The positive rates of methylation of Dactl promoter in renal cancer tissues and the corresponding control tissues were 70% and 6%,respectively. The positive rate in renal cancer tissues was significantly higher than that in the corresponding control tissues(P < 0.01). The correlation analysis of the silencing and down-regulation of Dactl gene and the methylated condition of Dactl promoter displayed statistical significance(P < O.05). Conclusion: The silencing or down-regulation of Dactl mRNA maybe participate in the renal carcinogenesis,and is related to the methylation of Dactl promoter.

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程 欢,王增军,苏建堂.肾透明细胞癌中β-环连蛋白抑制基因1启动子CpG岛甲基化与其失表达相关[J].南京医科大学学报(自然科学版),2011,(9):1266-1269

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  • 收稿日期:2011-03-29
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