Objective: To study the expression and distribution of ZBED3(zinc finger,BED-type containing 3) in mouse oocyte,and explore its role in oocyte maturation and early embryo development. Methods: The expressional level of ZBED3 mRNA in multipule tissues was analyzed using bioinformatic resources. Western blot and immunohistochemistry were used to detect the expression and distribution of ZBED3 in mouse oocyte. Results: The result of bioinformatic analysis showed that ZBED3 mRNA was specificly expressed in mouse oocyte and fertilized egg. Western blot confirmed the high-level expression of ZBED3 protein in mouse oocyte. And the result of immunohistochemistry showed the positive signal of ZBED3 was centralized in the cytoplasm of oocyte. Conclusion: ZBED3 maybe play roles in oocyte maturation and early embryo development.