多氯联苯暴露对斑马鱼脊柱形态及BMP-2?BMP-4基因表达的影响
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南京市科学技术发展指导性计划项目(20072D016)


Effects of embryonic exposure to polychlorinated biphenyls on zebrafish spinal morphology and the expression of BMP-2 and BMP-4 gene
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    摘要:

    目的:探讨环境有机污染物多氯联苯暴露对斑马鱼生存率-脊柱形态及骨形态发生蛋白(bone morphogenetic protein,BMP)-2-BMP-4基因表达的影响-方法:以斑马鱼为模式动物,配制0.125-1.000 mg/L 2个不同浓度的多氯联苯对斑马鱼胚胎进行暴露处理,同时设空白对照,观察多氯联苯暴露对斑马鱼幼鱼存活率-脊柱形态及BMP-2-BMP-4基因表达的影响-结果:①低浓度(0.125 mg/L)多氯联苯暴露120 hpf对斑马鱼幼鱼的存活率-脊柱形态无明显影响;②高浓度(1 mg/L)多氯联苯暴露120 hpf后,斑马鱼存活率明显下降且存活的斑马鱼大多出现了脊柱弯曲畸形的现象;③低-高浓度的多氯联苯暴露120 hpf后BMP-2及BMP-4基因表达均较空白对照组明显降低(P < 0.05)-结论:不同浓度的多氯联苯暴露对斑马鱼骨骼发育均有一定的毒害作用,提示我们必须加强对多氯联苯等环境有机污染物的监测和控制-

    Abstract:

    Objective: To study the effects of polychlorinated biphenyls on the skeletal development of zebrafish embryo and the expression of bone morphogenetic protein(BMP)-2 and BMP-4 genes. Methods: Zebrafish embryos were separately exposed to 0.125 mg/L,1.000 mg/L PCB1254 or pure water. Embryos were assessed for changes in embryonic survival and spinal deformity rate. BMP-2 and BMP-4 genes were assessed at 120 hours post-fertilization. Results: ① Low levels of polychlorinated biphenyls did not cause obvious changes of the survival or spinal deformity rate;② The survival rate declined and the spinal deformity rate increased obviously when zebrafish embryos were exposed to the high concentrations of PCBs(1 mg/L);③ The expression of BMP-2 and BMP-4 gene declined obviously in the PCB-treated larvae at concentrations 0.125 mg/L and 1.000 mg/L compared with their controls. Conclusion: Embryonic exposure to high levels of PCBs induced developmental deficit in zebrafish skeleton,which suggests the value of monitoring organic contaminant.

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鞠 黎,楼 跃,王艳萍,史春梅,朱冠忠,郭锡熔.多氯联苯暴露对斑马鱼脊柱形态及BMP-2?BMP-4基因表达的影响[J].南京医科大学学报(自然科学版),2011,(9):1277-1281

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  • 收稿日期:2011-03-15
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