大鼠胚胎肺泡发育形态学观察研究
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江苏省卫生厅项目基金(H200642)


Morphological research of lung development in fetal rat
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    目的:观察大鼠胚胎肺泡发育形态学变化,建立连续系统的胎肺发育形态学资料-方法:自SD大鼠孕16 d起,分别取孕16-19-21 d胎鼠肺脏行HE切片染色及透射电镜观察-结果:胚胎16 d胎肺开始出现上皮性管道,形成支气管众多分支,上皮细胞呈高柱状,围绕排列成环形;在胚胎19 d肺泡腔逐渐扩大,出现板层小体和2型肺泡上皮细胞;在胚胎21 d肺泡分隔增多,腔体扩张,间质变薄,同时板层小体数目增多-结论:大鼠胎肺发育是循序渐进的过程,一直延续到生后-板层小体的出现是肺泡2型上皮细胞分化的标志,且其数目随着发育而增多-

    Abstract:

    Objective: To study morphological evolution of fetal lung in SD rats and establish continuous systematic morphological datum. Methods: Fetal lungs at embryonic day16(E16),E19,E21,were taken out respectively,and were observed by HE staining under light microscope and electric microscope. Results: At E16,epithelial tract began to appear,which was constructed by tall epithelium,and developed many airway branches. At E19,alveolar space extended gradually,then lamellar body and type-II pneumonocytes began to emerge. At E21,alveolar separation increased gradually,and mesenchyma turned into thiner than before. At the same time the number of lamellar body increased gradually. Conclusion: The fetal lung development is a gradual process. In fact,this process will continue to postnatal stage. The appearance of lamellar body is the sign of differentiation of type-II pneumonocytes,and it increases along with lung development.

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杨 洋,周晓玉,郭 凯,阚 清,浦潇丹,郭锡熔.大鼠胚胎肺泡发育形态学观察研究[J].南京医科大学学报(自然科学版),2011,(9):1282-1284

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  • 收稿日期:2011-04-25
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